000			a
999			_c7791 _d7791
003			OSt
005			20181205131408.0
008			181205b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_96732 _aChoksi, P.
245			_aDevelopment of liquid chromatographic method for simuation determination of bromhexine hydrochloride and enrofloxacin in formulations
250			_aVol. 55 (02) February
260			_aMumbai _bIndian Drug Manufacture's Association - IDMA _c2018
300			_a44-49
520			_aA simple, specific, accurate, precise and reproducible method has been developed and validated for the estimation of bromhexine hydrochloride and enrofloxacin in fixed dose combination using RP-HPLC. The separation was achieved using stationary phase ODS Hypersil C18 column (250 mm× 4.6 mm i.d.) in isocratic mode, with mobile phase containing 0.05 M potassium dihydrogen phosphate buffer (pH 4 by o-phosphoric acid) : methanol: acetonitrile : triethylamine (40:20:40:01), at a flow rate of 1.0mL/min and eluents were monitored at 256 nm. The retention time of enrofloxacin and bromhexine HCl were found to be 3.00 min and 5.1 min respectively. The linearity for bromhexine HCl and enrofloxacin was in the range of 2-15 μg/mL and 20-150 μg/mL, respectively. The method was validated as per ICH guideline. The recoveries of bromhexine HCl and enrofloxacin were found in the range of 99.61-101.65% and 99.52-100.13 %, respectively. The method was successfully applied for the determination of both the drugs in combined dosage form.
650		0	_94639 _aPHARMACEUTICS
700			_96735 _aShaikh, F.
700			_96532 _aShah, D. A.
773	0		_dMumbai Indian Drug Manufactures Association _tIndian drugs
942			_2ddc _cAR