000			a
999			_c7855 _d7855
003			OSt
005			20181210104645.0
008			181210b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_96930 _aMishra, S. B.
245			_aDevelopment and validation of high-performance thin-layer chromatographic method for the determination of rutin from the aerial parts of Alhagi Pseudalhagi (M.BIEB) desv
250			_aVol. 55 (05) May
260			_aMumbai _bIndian Drug Manufacture's Association - IDMA _c2018
300			_a37-41
520			_aA simple, accurate and precise HPTLC method has been developed for the estimation of rutin from the aerial parts of Alhagi pseudalhagi (M. Bieb) Desv. The method employed silica gel 60 F254 precoated plates as stationary phase and a mixture of ethyl acetate: acetic acid: formic acid: water (10:1:1:1 v/v/v/v) as mobile phase. Densitometric scanning was performed at 366 nm after derivatizing the resolved peaks with anisaldehyde-sulphuric acid reagent. Beer’s law was obeyed in the concentration range of 400-1200ng/spot with regression equation for standard rutin as Y= 7.658x - 53.86 and r2=0.9885. The Retention factor for rutin is 0.39 ± 0.008. The % RSD (n=9) for interday, intraday precision and the recovery values were found to be 1.31% and 1.46% and 99.55-101.12%, respectively. The method was validated as per ICH Guidelines, proving its utility in estimation of rutin from the aerial parts of Alhagi pseudalhagi (M. Bieb) Desv.
650		0	_94639 _aPHARMACEUTICS
653			_aDensitometric
700			_96931 _aSingh, S.
700			_96932 _aMukerjee, A.
773	0		_dMumbai Indian Drug Manufactures Association _tIndian drugs
942			_2ddc _cAR