000			a
999			_c7940 _d7940
003			OSt
005			20190314104441.0
008			181221b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_97108 _aSuryadevara, V.
245			_aStability indicating RP - HPLC method development for the simultaneous estimation of lamivudine, tenofovir disoroxil fumarate and efavirenz in bulk and pharmaceutical formulation
250			_aVol. 55 (11) November
260			_aMumbai _bIndian Drug Manufacture's Association - IDMA _c2018
300			_a57-63
520			_aA simple, accurate reverse phase high performance liquid chromatographic method has been developed for the simultaneous estimation of lamivudine, tenofovir disoproxil fumarate and efavirenz in bulk and pharmaceutical formulations. The analytical method development was carried on Agilent make HPLC instrument using RP - C18 column. The mobile phase employed for the estimation is phosphate Buffer pH 4.0 :acetonitrile adjusted to pH 4.0 with glacial acetic acid which was pumped at a flow rate of 1.0 mL min-1 in the ratio of 42:58 v/v. the eluents were monitored at 260 nm. Linearity was obtained in the concentration range of 20-100 μg/mL of lamivudine, tenofovir disoproxil fumarate and 100-500 μg/mL efavirenz. Degradation studies shows that all the three drugs were not degraded under acidic, alkaline, thermal and photolytic conditions.The method was statistically validated and RSD was found to be within limits. Due to its simplicity, rapidness, high precision and accuracy, the proposed HPLC method can be applied for determining lamivudine, tenofovir disoproxil fumarate and efavirenz in bulk and in pharmaceutical dosage form
650		0	_94639 _aPHARMACEUTICS
700			_97109 _aSasidhar, R. L.
700			_97110 _aVenkateswara Rao, B.
773	0		_tIndian drugs _dMumbai Indian Drug Manufactures Association
856			_yClick here _uhttps://www.indiandrugsonline.org/issuesarticle-details?id=ODY2
942			_2ddc _cAR