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_97196 _aGupta, P. |
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| 245 | _aAntagonistic Properties of Methanol Extract of Elaeocarpus sphaericus Leaves against in vitro Growth, Germ Tube Formation, Adhesion and Biofilm of Candida Species | ||
| 250 | _aVol. 80(05), September-October | ||
| 260 |
_aMumbai _c2018 _bIndian Journal of Pharmaceutical Science |
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| 300 | _a949-954 | ||
| 520 | _aThe extracts of Elaeocarpus sphaericus (Roxb.) have shown antimicrobial activities against many pathogenic microbes including fungi. However, the effects of E. sphaericus have not been investigatd against key virulence attributes of Candida. In the present study, methanol extract of E. sphaericus leaves was analyzed against planktonic cells, hyphal growth, adhesion and biofilm formation of two most important fungal pathogens, Candida albicans and Candida glabrata. Methanol extract of E. sphaericus leaves was prepared by pressurized liquid extraction method. Inhibitory concentration of methanol extract of E. sphaericus leaves for both pathogens was determined by broth microdilution assay. Germ tube formation assay was performed to study the effect of the extract on hyphal transition. Effect of methanol extract of E. sphaericus leaves on adhesion and biofilm formation was investigated in microtiter plate using 2,3-bis(2-methoxy-4-nitro-sulfophenyl)-2H-tetrazolium-5-carboxanilide reduction assay. IC50 values of the extract for C. albicans and C. glabrata were found between 0.625-1.25 mg/ml. Serum-induced germ tube development in C. albicans was inhibited in presence of 5 mg/ml methanol extract . The extract remarkably inhibited in vitro adhesion and biofilm formation by C. albicans and C. glabrata in microtiter plates. The results of this study showed anticandida potential of methanol extract of E. sphaericus leaves. | ||
| 650 | 0 |
_94639 _aPHARMACEUTICS |
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| 653 | _aCandida glabrata | ||
| 653 | _aBiofilm | ||
| 653 | _aXTT assay | ||
| 653 | _aBroth microdilution | ||
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_97198 _aRastogi, A. |
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_97199 _aJoshi, S. |
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| 773 | 0 |
_tIndian journal of pharmaceutical sciences _dNew Delhi Indian Pharmaceutical Association _x0250-474X |
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| 856 |
_yClick here _uhttp://www.ijpsonline.com/articles/antagonistic-properties-of-methanol-extract-of-ielaeocarpus-sphaericusi-leaves-against-iin-vitroi-growth-germ-tube-formation-adhes-3551.html |
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