000			a
999			_c8476 _d8476
003			OSt
005			20190315095518.0
008			190308b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_97951 _aSiyuan, Su
245			_aProtein chip of boletus speciosus frost polysaccharide revealed the molecular mechanism of antitumor and immunositimulatory activities on macrophages
250			_aVol. 80(6), November-December
260			_aMumbai _c2018 _bIndian Journal of Pharmaceutical Science
300			_a1029-1038
520			_aThe polysaccharide of Boletus speciosus Frost has a backbone of (1→4)-α-L-mannopyranose residues, which branched at O-6 and the branches mainly comprised of (a→1)-α-D-galactopyranose residue. To find out signaling transduction pathways that mediate the effect of the polysaccharide of Boletus speciosus Frost on macrophages, the polysaccharide of B. speciosus Frost was used to stimulate the macrophages and 200 cytokines were detected with the help of a protein chip. Further analysis of the result revealed 48 up-regulated and 73 down-regulated cytokines. All cytokines were imported into the KEGG Pathway Database and NCBI to identify the signaling pathway and biological functions involved, respectively. The results showed that the cytokines fulfilled valuable functions in the JAK-STAT signaling pathway and NF-kappa B signaling pathway. This study thus revealed that the edible fungus polysaccharides could stimulate immune cells to secrete immune factors, which could provide a scientific basis for targeting sugar immunization preparations.
650		0	_94639 _aPHARMACEUTICS
700			_97954 _aWang, Meng
700			_97955 _aDing, Xiang
773	0		_x0250-474X _tIndian journal of pharmaceutical sciences _dNew Delhi Indian Pharmaceutical Association
856			_uhttp://www.ijpsonline.com/articles/protein-chip-of-iboletus-speciosusi-frost-polysaccharide-revealed-the-molecular-mechanism-of-antitumor-and-immunostimulatory-activ-3561.html _yClick here
942			_2ddc _cAR