000			a
999			_c8493 _d8493
003			OSt
005			20190315100819.0
008			190311b xxu||||| |||| 00| 0 eng d
040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_97980 _a Khangembam, Victoria C.
245			_aEvaluation of apoptosis inducing ability of parkia javanica seed extract in cancer cells
250			_aVol. 80(6), November-December
260			_aMumbai _c2018 _bIndian Journal of Pharmaceutical Science
300			_a1069-1077
520			_aBioactive compounds present in Parkia javanica seeds are reported to have anticancer effect. The present study was taken up to evaluate the apoptosis inducing ability of methanol extract of Parkia javanica seeds in cancer cells. The extract was found to cause 50 % cell death in HeLa and MCF-7 cells at 0.54 and 0.74 mg/ml, respectively, which is lower than that of normal healthy cells. In haematoxylin-eosin and fluorescent staining of the extract-treated cancer cells, chromatin condensation and nuclear fragmentation were observed. Fluorescent-activated cell sorting analysis using Annexin V-fluorescein isothiocyanate/propidium iodide also revealed the presence of apoptotic and secondary necrotic cell populations in the extract-treated cancer cells. In gel electrophoresis, DNA fragmentation, a biochemical hallmark of apoptosis was also observed. Caspase-3, an enzyme activated both in extrinsic and intrinsic pathways of apoptosis was detected in extract-treated HeLa cells by immunocytochemistry. The results of the present study suggested that Parkia javanica seed has the ability to induce apoptosis in cancer cells and could be considered in future endeavours for development of safer natural anticancer agent.
650		0	_94639 _aPHARMACEUTICS
700			_97981 _a Srivastava, S. K.
700			_97982 _aGeeta, D.
773	0		_dNew Delhi Indian Pharmaceutical Association _x0250-474X _tIndian journal of pharmaceutical sciences
856			_uhttp://www.ijpsonline.com/articles/evaluation-of-apoptosis-inducing-ability-of-iparkia-javanicai-seed-extract-in-cancer-cells-3565.html _yClick here
942			_2ddc _cAR