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040			_aAIKTC-KRRC _cAIKTC-KRRC
100			_98019 _a Bandla, Jahnavi
245			_aDevelopment and validatiion of a stability-indicationg method for the simultaneous estimation of sofosbuvir and ledipasvir by RP-HPLC
250			_aVol. 88(6), November-December
260			_aMumbai _c2018 _bIndian Journal of Pharmaceutical Science
300			_a1170-1176
520			_aA stability indicating reversed-phase high-performance liquid chromatography method was developed and validated for simultaneous quantification of sofosbuvir and ledipasvir in tablets. The chromatographic separation was done in an isocratic mode using the Discovery C18 (250×4.6 mm, 5 µ particle size) column. The mobile phase 0.1 % ortho phosphoric acid and acetonitrile 45:55 (% v/v) at a flow rate of 1.0 ml/min and at ambient temperature was used. The wavelength used for detection was 270 nm. The retention time for sofosbuvir was found to 2.08 min and that of ledipasvir was 3.06 min. Sofosbuvir and ledipasvir were linear in the concentration ranges of 100 to 600 µg/ml and 22.5 to 135 µg/ml, respectively. The developed method was validated and found to be accurate, specific and robust. Both the drugs were subjected to the stressed conditions like acidic, basic, oxidative, photolytic and thermal conditions. The degradation results were found satisfactory. This method could be applied for the simultaneous estimation of sofosbuvir and ledipasvir in tablets.
650		0	_94639 _aPHARMACEUTICS
700			_98020 _aGanpaty, S.
773	0		_x0250-474X _dNew Delhi Indian Pharmaceutical Association _tIndian journal of pharmaceutical sciences
856			_uhttp://www.ijpsonline.com/articles/development-and-validation-of-a-stabilityindicating-method-for-the-simultaneous-estimation-of-sofosbuvir-and-ledipasvir-by-rphplc-3579.html _yClick here
942			_2ddc _cAR