| 000 | a | ||
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| 999 |
_c9563 _d9563 |
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| 003 | OSt | ||
| 005 | 20190930150439.0 | ||
| 008 | 190930b xxu||||| |||| 00| 0 eng d | ||
| 040 |
_aAIKTC-KRRC _cAIKTC-KRRC |
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| 100 |
_99665 _aAlrabie, Ali |
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| 245 | _aGC-MS Analysis, HPTLC fingerprint profile and DPPH free radical scavenging assay of methanol extract of martynia annua linn seeds | ||
| 250 | _aVol. 11 (06) | ||
| 260 |
_aBhopal _bInnovare Academic Sciences Pvt Ltd _c2019 |
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| 300 | _a14-22p. | ||
| 520 | _aTo investigate the photochemical constituents present in methanol extract of martynia annua seeds using Gas Chromatography-Mass Spectroscopy(GC-MS), High-Performance Thin–Layer Chromatography(HPTLC) analysis and study antioxidant activity. Methods: Methanol extract of Martynia annua seeds were subjected to GC-MS and HPTLC analysis. HPTLC analysis was carried out using GAMAG system with a linomate5 applicator, system mobile phase (Toluene: Chloroform: Ethanol (4:4:1 V/V/V)), two different volume of extract was applied 2 µl and 5 µl. GC-MS analysis was carried out on JEOL GC MATE ΙΙ, column HP 5 MS and Quadruple double focusing mass analyzer. Antioxidant activity was determined by DPPH assay. Results: GC-MS analysis provided 17 peaks indicating the presence of seventeen different phytochemicals in methanol extract of martynia annua seeds. HPTLC fingerprint showed 6 peaks at both size 2 µl and 5 µl at 254 nm whereas 4 peaks, 9 peaks were detected at 366 nm for 2 µl and 5 µl respectively. After derivatization with 10 % methanolic sulphuric acid, 8 peaks, 11 peaks were detected for 2 µl and 5 µl respectively when the derivatized plate was scanned at 540 nm. DPPH free radical scavenging result showed EC50 value of 44.1±1.1 µg/ml. Conclusion: The GC-MS analysis showed the presence of fatty acids, ester, aldehydes and ketones whereas in HPTLC different peaks at different UV-lights before and after derivatization were observed. Maximum percentage inhibition using DPPH assay was found 74 at concentration of 50 µg/ml. | ||
| 650 | 0 |
_94639 _aPHARMACEUTICS |
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| 700 |
_99666 _aOla Bassar |
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| 773 | 0 |
_x0975 – 1491 _dBhopal Innovare Academic Sciences Pvt Ltd _tInternational journal of pharmacy and pharmaceutical science |
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| 856 |
_uhttps://innovareacademics.in/journals/index.php/ijpps/article/view/32206 _yClick here |
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| 942 |
_2ddc _cAR |
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