| 000 | a | ||
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| 999 |
_c9795 _d9795 |
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| 003 | OSt | ||
| 005 | 20191019145451.0 | ||
| 008 | 191019b xxu||||| |||| 00| 0 eng d | ||
| 040 |
_aAIKTC-KRRC _cAIKTC-KRRC |
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| 100 |
_910010 _aManikandan, S. |
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| 245 | _aAnti-tumour activity of bromopyrrole alkaloids against human breast tumour (MCF-7) through apoptosis induction | ||
| 250 | _aVol. 11 (06) | ||
| 260 |
_aBhopal _bInnovare Academic Sciences Pvt Ltd _c2019 |
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| 300 | _a94-100 | ||
| 520 | _aThe present study was conducted to screen the cytotoxicity and initiation of apoptosis in MCF-7 human breast tumour cells using the Indian sponge Acanthostylotela cornuta of the Gulf of Mannar. Methods: The crude methanol extract of A. cornuta was fractionated using a reversed phase silica gel column chromatography. The purity of the active fraction of bromopyrrole alkaloids was confirmed with Gas Chromatography-Mass Spectrometry (GCMS). The bromopyrrole alkaloids induce apoptotic changes in MCF-7 cells were studied with electrophoresis, caspase assay, and different staining analysis. The MCF-7 cells were analyzed by Flow cytometry to determine their DNA content. Results: Bromopyrrole alkaloids of A. cornuta showed cytotoxicity against MCF-7 human breast tumour cells with the IC50 value of 8.0μg/ml. The bromopyrrole compounds induced cells exhibited scatted red fluorescence, showing the presence of several residual bodies and condensation of chromatin. Caspase-3, pro-caspase-9, caspase-9 and Poly-ADP-Ribose-Polymerase (PARP) activity were occurred in bromopyrrole alkaloids treated tumour cell. The cell cycle arrest is conceivable that the compound prevented the progression of cell cycle through the G phase resulting from inhibition of survival, leading them to undergo apoptosis. Conclusion: Bromopyrrole alkaloids of A. cornuta possess antitumor activity which was arrested the G phase in cell cycle that clearly indicated its nature as that of antitumour drugs. | ||
| 650 | 0 |
_94639 _aPHARMACEUTICS |
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| 700 |
_910011 _aGanesapandian, S. |
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| 773 | 0 |
_x2656-0097 _tInternational journal of pharmacy and pharmaceutical science _dBhopal Innovare Academic Sciences Pvt Ltd |
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| 856 |
_uhttps://innovareacademics.in/journals/index.php/ijpps/article/view/29092 _yClick here |
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| 942 |
_2ddc _cAR |
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