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040 _aAIKTC-KRRC
_cAIKTC-KRRC
100 _910152
_aSharma, Hemraj
245 _aComparative analysis of vitamin-c concentration in commercial fruit juices and fresh fruits of nepal with effect of temperature
250 _aVol. 11(8)
260 _aBhopal
_bInnovare Academic Sciences Pvt Ltd
_c2019
300 _a46-51p.
520 _aObjective: It was aimed to determine the best storage temperature for fruits to achieve the highest quality of vitamin-C and to compare vitamin C concentration between commercial fruits juices and fresh fruit juices in by using Spectrophotometry and Titrimetric method Methods: Titration involved the redox reaction between iodine and vitamin C. As the iodine was added during the titration, the ascorbic acid was oxidized to dehydroascorbic acid, while the iodine was reduced to iodide ions. The Spectrophotometric method involved the coupling reaction of 2,4 dinitrophenylhydrazine (DNPH) dye with Vitamin C. The samples were analyzed using UV-Vis-Spectrophotometer at 521 nm. Results: Titrimetric and spectroscopic methods were performed for fresh and marketed fruits comprising of apple, grapes, lemon, orange. The maximum amount was found in lemon and orange whereas apple and grapes contained lesser amount of Vitamin C. The stability of marketed fruit juices made up of apple, grapes, lemon, orange were analyzed by storing them on the freeze at 0 °C and 10 °C and on the hot air oven at 20 °C, 30 °C, 40 °C, 50 °C for 72 h and estimated by both Titrimetric and Spectroscopy method. UV-Spectroscopy method showed that, at freezing condition up to 10 °C temperature, degradation was too low but when the temperature reached 50 °C the extent of degradation was more, showing 24.56% apple juice, 10.89% orange juice,12.70% grapes juice and 50% orange juice were degraded in 100 ml sample. Similar results were observed by analysing the samples with titration technique. Conclusion: A new analytical method was developed to address the content of vitamin C in fruits consumed in the local market of Nepal along with the best possible storage of fruit juice to yield the maximum amount of nutrients. Keywords: Vitamin C, Temperature, UV-Spectroscopy, Titration, Phytochemical, TLC
650 0 _94639
_aPHARMACEUTICS
700 _910154
_aSapkota, Hari Prasad
773 0 _tInternational journal of pharmacy and pharmaceutical science
_dBhopal Innovare Academic Sciences Pvt Ltd
_x2656-0097
856 _uhttps://innovareacademics.in/journals/index.php/ijpps/article/view/33408
_yClick here
942 _2ddc
_cAR