| 000 | a | ||
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| 999 |
_c9921 _d9921 |
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| 003 | OSt | ||
| 005 | 20191101113159.0 | ||
| 008 | 191101b xxu||||| |||| 00| 0 eng d | ||
| 040 |
_aAIKTC-KRRC _cAIKTC-KRRC |
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| 100 |
_910219 _aKapoor, Manish |
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| 245 | _aDetermination of vindoline and rutin content in five different morphotypes of catharanthus roseus leaves using HPLC | ||
| 250 | _aVol. 11(8) | ||
| 260 |
_aBhopal _bInnovare Academic Sciences Pvt Ltd _c2019 |
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| 300 | _a52-57p. | ||
| 520 | _aObjective: To determine total phenolic and flavonoids contents and also quantify vindoline and rutin in different morphotypes of Catharanthus roseus using High-performance liquid chromatography (HPLC) method. Methods: Total flavonoids content (TFC) was determined by Aluminium chloride colorimetric and total phenolic content (TPC) was estimated by Folin-Ciocalteu reagent assay. The chromatographic separation was done by using a C18 column at room temperature and eluted with a mobile phase consisting of a mixture of phosphate buffer (pH=5.8) and acetonitrile at a flow rate of 1.0 ml/minute and detection was carried out at 254 nm. Results: TPC and TFC content was found highest in Cr00DP and lowest in Cr00WFSRE. Results also showed that the purple morphotypes Cr00DP gives more vindoline (0.3 mg/g) and rutin (18.57 mg/g) concentration compared to the pink morphotype Cr00PFRE contained 18.3 mg/g rutin and 0.2 mg/g vindoline. White morphotypes contained 0.383 mg/g rutin and 0.004 mg/g vindoline which was significantly less as compared to purple and pink morphotypes. Conclusion: The plant has significant number of alkaloids and flavonoids. The obtained outcomes from different morphotypes are thus significant for the purpose of vindoline and rutin isolation from Catharanthus roseus plant. These isolated bioactive phytoconstituents are a good candidate for further pharmacological and clinical study. | ||
| 650 | 0 |
_94639 _aPHARMACEUTICS |
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| 700 |
_910220 _aRani, Jyoti |
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| 773 | 0 |
_dBhopal Innovare Academic Sciences Pvt Ltd _x2656-0097 _tInternational journal of pharmacy and pharmaceutical science |
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| 856 |
_uhttps://innovareacademics.in/journals/index.php/ijpps/article/view/31140 _yClick here |
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| 942 |
_2ddc _cAR |
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