INNOVATIVE METHOD DEVELOPMENT AND FORCED DEGRADATION STUDIES FOR SIMULTANEOUS ESTIMATION OF SOFOSBUVIR AND LEDIPASVIR BY RP HPLC
Reddy, B. Anjaneyulu
INNOVATIVE METHOD DEVELOPMENT AND FORCED DEGRADATION STUDIES FOR SIMULTANEOUS ESTIMATION OF SOFOSBUVIR AND LEDIPASVIR BY RP HPLC - Vol.11(2) - M P Innovare Academic Sciences Pvt Ltd 2019 - 34-41p.
Objective:
To develop an innovative, rapid, simple, cost
-effective
, stability indicating reverse phase
-high performance liquid chromatography (RP
-
HPLC) method for simultaneous estimation of ledipasvir (LP) and sofosbuvir (SB) in combination pill dosage form.
Method
s:
The method was developed using C8 column, 250
mm
x 4.6 mm, 5
mm
using mobile section comprising of 0.1%
(v/v)
orthophosphoric
acid buffer
at
pH 2.2
and acetonitrile in the ratio of 45:55 that was pumped through the column at a flow rate of 0.8 ml/min. Temperature
was
maintained at 30
°C, the effluents w
ere monitored at 260 nm with the help of usage of PDA detector.
Result
s:
The retention time of LP and SB were found to be 2.246 min and 3.502 min.
The approach was found to be linear with the variety of 9
-36
μg
/ml
and 40
-
240 μg
/ml
for LP and SB respectively, the assay of estimated compounds were found to be 99.65% and 99.73% w/v for LP and SB
respectively.
Conclusio
n:
The pressured samples changed into analyzed and this proposed a technique turned into determined to be particular and stability
indicating as no interfering peaks of decay compound and excipients were observed. Hence, the approach was easy and economica
l that may be
efficiently applied for simultaneous estimation of both LP and SB in bulk and combination tablet system
PHARMACEUTICS
INNOVATIVE METHOD DEVELOPMENT AND FORCED DEGRADATION STUDIES FOR SIMULTANEOUS ESTIMATION OF SOFOSBUVIR AND LEDIPASVIR BY RP HPLC - Vol.11(2) - M P Innovare Academic Sciences Pvt Ltd 2019 - 34-41p.
Objective:
To develop an innovative, rapid, simple, cost
-effective
, stability indicating reverse phase
-high performance liquid chromatography (RP
-
HPLC) method for simultaneous estimation of ledipasvir (LP) and sofosbuvir (SB) in combination pill dosage form.
Method
s:
The method was developed using C8 column, 250
mm
x 4.6 mm, 5
mm
using mobile section comprising of 0.1%
(v/v)
orthophosphoric
acid buffer
at
pH 2.2
and acetonitrile in the ratio of 45:55 that was pumped through the column at a flow rate of 0.8 ml/min. Temperature
was
maintained at 30
°C, the effluents w
ere monitored at 260 nm with the help of usage of PDA detector.
Result
s:
The retention time of LP and SB were found to be 2.246 min and 3.502 min.
The approach was found to be linear with the variety of 9
-36
μg
/ml
and 40
-
240 μg
/ml
for LP and SB respectively, the assay of estimated compounds were found to be 99.65% and 99.73% w/v for LP and SB
respectively.
Conclusio
n:
The pressured samples changed into analyzed and this proposed a technique turned into determined to be particular and stability
indicating as no interfering peaks of decay compound and excipients were observed. Hence, the approach was easy and economica
l that may be
efficiently applied for simultaneous estimation of both LP and SB in bulk and combination tablet system
PHARMACEUTICS