IN VITRO ANTIOXIDANT AND FREE RADICAL SCAVENGING POTENTIAL OF METHANOLIC EXTRACTS OF UVARIA CHAMAE LEAVES AND ROOTS
Nwakaego, Nwokolo Lorreta
IN VITRO ANTIOXIDANT AND FREE RADICAL SCAVENGING POTENTIAL OF METHANOLIC EXTRACTS OF UVARIA CHAMAE LEAVES AND ROOTS - Vol.11(1) - M P Innovare Academic Sciences Pvt Ltd 2019 - 67-71p.
Objective:
This study investigated the
in vitro
antioxidant and free radical potentials of methanol extracts of
Uvaria chamae
leaves and roots
.
Method
s:
Fresh
Uvaria chamae
leaves and roots were air dried, pulverized and extracted using methanol. Phytochemical, total phenolic, flavonoids
,
antioxidant
and tannin contents
, DPPH, hydroxyl, and superoxide rad
ical scavenging properties
of the extracts were determined using standard methods.
Result
s:
In vitro
antioxid
ant potentials revealed that
methanol extract of
Uvaria chamae
leaves
contains vitamin A
(4871±79.21 I
. U
) and vitamin C
(1.72±0.02%) while the root extract contains vitamin A (673.28±0.00I
. U
) and vitamin C (1.66±0.01%). Both extracts had equal contents of vitamin
E (8.83±0.04 mg/100g). The leaf extract scavenged 1,1
-diphenyl
-2-picrylhydrazyl
radical (DPPH) in a concentration dependent manner with the
correlation coefficient (R
2
) of 0.839 and effective concentration (EC
50
) of
31.19 μg/ml
, while the root extract scavenged DPPH with R
2
of0.778 and
EC
50
of
14.00 μg
/ml
. The leaf and root extracts scavenged superoxide radical and hydroxyl radical with EC
50
of
5.93 μg/ml
and 719.45 μg/ml;
107.89 μg/ml
and 912.01 μg
/ml
respectively compared to the
EC
50
of ascorbic standard (
30.27 μg/ml
) and
EC
50
Conclusio
n:
The
leaves and roots of
Uvaria
chamae
are
rich in natural antioxidants that can be exploited in the treatment of diseases related to
oxidative stress.
of vitamin E standard
(106.66μg
/ml
) respectively. The
leaf extract showed significantly higher (
p
<0.05) anti radical power (ARP) of superoxide (0.17) comp
PHARMACEUTICS
IN VITRO ANTIOXIDANT AND FREE RADICAL SCAVENGING POTENTIAL OF METHANOLIC EXTRACTS OF UVARIA CHAMAE LEAVES AND ROOTS - Vol.11(1) - M P Innovare Academic Sciences Pvt Ltd 2019 - 67-71p.
Objective:
This study investigated the
in vitro
antioxidant and free radical potentials of methanol extracts of
Uvaria chamae
leaves and roots
.
Method
s:
Fresh
Uvaria chamae
leaves and roots were air dried, pulverized and extracted using methanol. Phytochemical, total phenolic, flavonoids
,
antioxidant
and tannin contents
, DPPH, hydroxyl, and superoxide rad
ical scavenging properties
of the extracts were determined using standard methods.
Result
s:
In vitro
antioxid
ant potentials revealed that
methanol extract of
Uvaria chamae
leaves
contains vitamin A
(4871±79.21 I
. U
) and vitamin C
(1.72±0.02%) while the root extract contains vitamin A (673.28±0.00I
. U
) and vitamin C (1.66±0.01%). Both extracts had equal contents of vitamin
E (8.83±0.04 mg/100g). The leaf extract scavenged 1,1
-diphenyl
-2-picrylhydrazyl
radical (DPPH) in a concentration dependent manner with the
correlation coefficient (R
2
) of 0.839 and effective concentration (EC
50
) of
31.19 μg/ml
, while the root extract scavenged DPPH with R
2
of0.778 and
EC
50
of
14.00 μg
/ml
. The leaf and root extracts scavenged superoxide radical and hydroxyl radical with EC
50
of
5.93 μg/ml
and 719.45 μg/ml;
107.89 μg/ml
and 912.01 μg
/ml
respectively compared to the
EC
50
of ascorbic standard (
30.27 μg/ml
) and
EC
50
Conclusio
n:
The
leaves and roots of
Uvaria
chamae
are
rich in natural antioxidants that can be exploited in the treatment of diseases related to
oxidative stress.
of vitamin E standard
(106.66μg
/ml
) respectively. The
leaf extract showed significantly higher (
p
<0.05) anti radical power (ARP) of superoxide (0.17) comp
PHARMACEUTICS