Determination of in vitro human whole blood‑to‑plasma ratio of THJ‑018 utilizing gas chromatography–Mass spectrometry
Sachil Kumar
Determination of in vitro human whole blood‑to‑plasma ratio of THJ‑018 utilizing gas chromatography–Mass spectrometry - Vol.53(1), Jan-Feb - Mumbai Wolter Kluwer 2021 - 13-18p.
OBJECTIVES: This study was aimed to determine in vitro human whole blood‑to‑plasma ratio (KWB/P)
of THJ‑018 by gas chromatography/mass spectrometry (GC/MS).
MATERIALS AND METHODS: The samples (human blood) were sprayed with THJ‑018 and an
internal standard and extracted using solid‑phase extraction. THJ‑018 was determined in the final
extracts by GC/MS.
RESULTS: The value for K WB/P was 1.56 (1.38–1.81), and red blood cell partitioning was
1.01 (1.01–1.02). The distribution of THJ‑018 between whole blood and plasma was observed to
be affected by temperature.
CONCLUSION: The data analysis supports the proposition that the ratio of the plasma to whole blood
concentrations (1.56) is a suitable parameter characterizing THJ‑018 distribution in whole blood. For
toxicological analysis, it would be best to refrain from converting any drug concentration measured
in whole blood to that anticipated in plasma or serum; however, toxic and therapeutic concentrations
should be determined for the individual specimens collected.
PHARMACOLOGY
Determination of in vitro human whole blood‑to‑plasma ratio of THJ‑018 utilizing gas chromatography–Mass spectrometry - Vol.53(1), Jan-Feb - Mumbai Wolter Kluwer 2021 - 13-18p.
OBJECTIVES: This study was aimed to determine in vitro human whole blood‑to‑plasma ratio (KWB/P)
of THJ‑018 by gas chromatography/mass spectrometry (GC/MS).
MATERIALS AND METHODS: The samples (human blood) were sprayed with THJ‑018 and an
internal standard and extracted using solid‑phase extraction. THJ‑018 was determined in the final
extracts by GC/MS.
RESULTS: The value for K WB/P was 1.56 (1.38–1.81), and red blood cell partitioning was
1.01 (1.01–1.02). The distribution of THJ‑018 between whole blood and plasma was observed to
be affected by temperature.
CONCLUSION: The data analysis supports the proposition that the ratio of the plasma to whole blood
concentrations (1.56) is a suitable parameter characterizing THJ‑018 distribution in whole blood. For
toxicological analysis, it would be best to refrain from converting any drug concentration measured
in whole blood to that anticipated in plasma or serum; however, toxic and therapeutic concentrations
should be determined for the individual specimens collected.
PHARMACOLOGY