Development and validation of a precise RP- HPLC method to determine gentiopicroside content in cultures of gentiana kurroo royle
Alphonse, Mariadoss
Development and validation of a precise RP- HPLC method to determine gentiopicroside content in cultures of gentiana kurroo royle - Vol.56(1), Jan-Mar - Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022 - 264-271p.
Background: Gentipicroside (GPD) is a major bioactive seco-iridoid glycoside in the
methanolic extracts of roots and rhizomes of
Gentiana kurroo Royle. GPD has anti-
inflammatory, antidiabetic, analgesic, antinociceptive, antibacterial and free radical
scavenging activities. Although this compound was analyzed by various methods in
different
Gentiana species previously, no valid method was documented describing
the accuracy and precision for the detection and quantification of GPD from
in vitro
samples of
G. kurroo. Materials and Methods: A simple, accurate and highly sensitive
reversed-phase high-performance liquid chromatographic (RP-HPLC) method was
developed and validated according to the International Conference on Harmonization
(ICH) guidelines. Results: Methanol and 0.1% acetic acid in millipore water [30:70, (v/v)]
was used as a mobile phase and eluted at an isocratic flow rate of 1.0 ml/min under
room temperature. The calibration curve was linear in the concentration range of 10-100
μg/ml with a correlation coefficient of 0.9967. The relative standard deviation (RSD)
of repeatability and inter-day precision was less than 2%. The limit of detection (LOD)
and limit of quantification (LOQ) of GPD was 0.083 μg/ml and 0.25 μg/ml, respectively.
Recoveries from
in vitro samples ranged from 91.0 to 114.0% and the precision of the
method in terms of retention time (%RSD ≤ 2.01) and peak area (% RSD ≤5.11) were
satisfactory. Conclusion: The validated RP-HPLC- PDA method can be used routinely for
the determination of GPD in
in vitro cultures and in vivo plants of
G. kurroo.
PHARMACEUTICS
Development and validation of a precise RP- HPLC method to determine gentiopicroside content in cultures of gentiana kurroo royle - Vol.56(1), Jan-Mar - Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022 - 264-271p.
Background: Gentipicroside (GPD) is a major bioactive seco-iridoid glycoside in the
methanolic extracts of roots and rhizomes of
Gentiana kurroo Royle. GPD has anti-
inflammatory, antidiabetic, analgesic, antinociceptive, antibacterial and free radical
scavenging activities. Although this compound was analyzed by various methods in
different
Gentiana species previously, no valid method was documented describing
the accuracy and precision for the detection and quantification of GPD from
in vitro
samples of
G. kurroo. Materials and Methods: A simple, accurate and highly sensitive
reversed-phase high-performance liquid chromatographic (RP-HPLC) method was
developed and validated according to the International Conference on Harmonization
(ICH) guidelines. Results: Methanol and 0.1% acetic acid in millipore water [30:70, (v/v)]
was used as a mobile phase and eluted at an isocratic flow rate of 1.0 ml/min under
room temperature. The calibration curve was linear in the concentration range of 10-100
μg/ml with a correlation coefficient of 0.9967. The relative standard deviation (RSD)
of repeatability and inter-day precision was less than 2%. The limit of detection (LOD)
and limit of quantification (LOQ) of GPD was 0.083 μg/ml and 0.25 μg/ml, respectively.
Recoveries from
in vitro samples ranged from 91.0 to 114.0% and the precision of the
method in terms of retention time (%RSD ≤ 2.01) and peak area (% RSD ≤5.11) were
satisfactory. Conclusion: The validated RP-HPLC- PDA method can be used routinely for
the determination of GPD in
in vitro cultures and in vivo plants of
G. kurroo.
PHARMACEUTICS