Validation of new liquid chromatographic method for naturally isolated quercetin and its application to ayurvedic formulation
Dinnimath, Basavaraj Mrutyunjay
Validation of new liquid chromatographic method for naturally isolated quercetin and its application to ayurvedic formulation - Vol. 56 (06) - Mumbai Indian Drug Manufacture's Association - IDMA 2019 - 46-50
Quercetin was isolated from Aerva lanata (L) and investigated for method development. A simple, accu- rate, reproducible method was developed using RP-HPLC. Commercial formulation of Quercetin 250mg capsules (Quercetin Complex-Solgar, USA) was used followed by an Ayurvedic proprietary medicine for the study. The chromatographic separation was performed on Shiseido Capcell Pak C 18 column (250 X 4.6 mm, 5 μm), mobile phase developed was 0.3% formic acid:acetonitrile:methanol (40:20:40) at the flow rate of 10μL/min at 22 0 C. The detector used was PDA with the detection wavelength of 370 nm. R T was 4.9mn with LOD-0.816 μg/ml and LOQ-2.473 μg/ml. The correlation coefficient was 0.992%. The linearity range was observed in the concentration range of 2-20 μg/mL and the recovery was found to be 99.91 to 102.65 %. Finally, the method applied to KACHCHNAR Ayurvedic formulation justified with characteristic peak for identifying quercetin in it.
PHARMACEUTICS
Validation of new liquid chromatographic method for naturally isolated quercetin and its application to ayurvedic formulation - Vol. 56 (06) - Mumbai Indian Drug Manufacture's Association - IDMA 2019 - 46-50
Quercetin was isolated from Aerva lanata (L) and investigated for method development. A simple, accu- rate, reproducible method was developed using RP-HPLC. Commercial formulation of Quercetin 250mg capsules (Quercetin Complex-Solgar, USA) was used followed by an Ayurvedic proprietary medicine for the study. The chromatographic separation was performed on Shiseido Capcell Pak C 18 column (250 X 4.6 mm, 5 μm), mobile phase developed was 0.3% formic acid:acetonitrile:methanol (40:20:40) at the flow rate of 10μL/min at 22 0 C. The detector used was PDA with the detection wavelength of 370 nm. R T was 4.9mn with LOD-0.816 μg/ml and LOQ-2.473 μg/ml. The correlation coefficient was 0.992%. The linearity range was observed in the concentration range of 2-20 μg/mL and the recovery was found to be 99.91 to 102.65 %. Finally, the method applied to KACHCHNAR Ayurvedic formulation justified with characteristic peak for identifying quercetin in it.
PHARMACEUTICS