Study of inhibitory potential and percent inhibition of oil of Syzygiumaromaticum and leaves of Ocimum sanctum on ESBL enzyme from Escherichia coli in broilers of Jabalpur (Record no. 11049)

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005 - DATE AND TIME OF LATEST TRANSACTION
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fixed length control field 200124b xxu||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency AIKTC-KRRC
Transcribing agency AIKTC-KRRC
100 ## - MAIN ENTRY--PERSONAL NAME
9 (RLIN) 11914
Author Shrivastav, Arpita
245 ## - TITLE STATEMENT
Title Study of inhibitory potential and percent inhibition of oil of Syzygiumaromaticum and leaves of Ocimum sanctum on ESBL enzyme from Escherichia coli in broilers of Jabalpur
250 ## - EDITION STATEMENT
Volume, Issue number Vol.51(5), Sep-Oct
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Mumbai
Name of publisher, distributor, etc. Wolter Kluwer
Year 2019
300 ## - PHYSICAL DESCRIPTION
Pagination 337-342p.
520 ## - SUMMARY, ETC.
Summary, etc. OBJECTIVE: The inhibitory potential and percent inhibition of Syzygiumaromaticum oil and fresh juice of Ocimumsanctum leaves on beta-lactamase enzyme of cecal samples of healthy broilers were studied on samples phenotypically positive for extended-spectrum beta-lactamase (ESBL)-producing Escherichiacoli.MATERIALS AND METHODS: Four hundred cecal samples screened for ESBL-producing E. coli were collected from 38 poultry sale outlets located in Jabalpur. The effect of S.aromaticumoiland O.sanctum leaves was seen by colorimetric assay with CENTA and Nitrocefin as chromogenic substrate.RESULTS: Mean absorbance value was inversely propotional to the inhibitory potential. Syzigium aromaticum exhibited 0.4±0.02 and 0.41±0.03 mean absorbance value, 28 per cent and 27 per cent of inhibition with CENTA and Nitrocefin respectively. Ocimum sanctum mean absorbance value and per cent inhibition with CENTA and Nitrocefin was 2.03±0.02 and 10.0 ; 1.97±0.06 and 10.0 respectively (p>0.05) showing non- significant difference in CENTA and Nitrocefin activity. Tazobactum (100 μM) as standard control exhibited a mean absorbance value of 0.12 ± 0.01 and 0.13 ± 0.01 and percent inhibition of 99.88 and 98 against CENTA and Nitrocefin, respectively. Combination of Ocimum sanctum and Syzigium aromaticum showed range of 1.69±0.05 to 1.90±0.08 1.61±0.06 to 1.92±0.08 of absorbance value with per cent inhibition of 14 to 15.9 with CENTA and Nitrocefin respectively.CONCLUSION: The results depicted that the inhibition of beta-lactamase enzyme activity with S.aromaticum oil was higher than that of O.sanctum leaf juice, and combination of both the herbs showed not much difference in activity.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 4774
Topical term or geographic name entry element PHARMACOLOGY
700 ## - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 4035
Co-Author Sharma, R. K.
773 0# - HOST ITEM ENTRY
Title Indian Journal of Pharmacology
Place, publisher, and date of publication Andheri - Mumbai Wolters Kluwer India Private Limited
International Standard Serial Number 0253-7613
856 ## - ELECTRONIC LOCATION AND ACCESS
URL http://www.ijp-online.com/temp/IndianJPharmacol515337-1885807_051418.pdf
Link text Click here
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