Apoptosis induction in cancer cell lines by the carotenoid Fucoxanthinol from Pseudomonas stutzeri JGI 52 (Record no. 11065)
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| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20200206112007.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 200206b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | AIKTC-KRRC |
| Transcribing agency | AIKTC-KRRC |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 11940 |
| Author | Shukla, Megha |
| 245 ## - TITLE STATEMENT | |
| Title | Apoptosis induction in cancer cell lines by the carotenoid Fucoxanthinol from Pseudomonas stutzeri JGI 52 |
| 250 ## - EDITION STATEMENT | |
| Volume, Issue number | Vol.50(3), May-June |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Mumbai |
| Name of publisher, distributor, etc. | Wolter Kluwer |
| Year | 2018 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Pagination | 116-122p. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | CONTEXT: Microorganisms produce a variety of pigments and many pigments from bacteria were reported to have therapeutic potential including anticancer effects.AIM: The aim of this study is to evaluate the anticancer potential a yellow pigment from newly isolated Pseudomonasstutzeri JGI 52.MATERIALS AND METHODS: Serial dilution method was adopted for the isolation of pigmented bacteria from soil sources. Pigment extraction was carried out from bacterial isolates using methanol as the solvent and the pigment was purified by thin layer chromatography. Through 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay, the effect of the pigment fraction on cancer cells was analyzed. Apoptosis induction was evaluated by caspase‑3 activity assay, DNA fragmentation analysis, cell morphology observation by AO‑EB staining under the fluorescence microscope, and cellular cytotoxicity was analysed by lactate dehydrogenase (LDH) release assay. Characterization of the purified pigment was by high‑performance liquid chromatography and electrospray ionization‑mass spectrometry analysis.STATISTICAL ANALYSIS: Significance of the results was confirmed by performing one‑way analysis of variance.RESULTS: The pigment (PY3) from P.stutzeri inhibited the proliferation of HeLa, HepG2, and Jurkat cells and found to be less toxic to lymphocytes and CHO cells. PY3 exhibited apoptotic potential in the cancer cell lines, as evidenced by cleavage of DNA, LDH release, activation of caspase‑3, and decrease in cell count. Results of mass spectra indicated the presence of “fucoxanthinol” which was earlier reported as an anticancer compound from seaweeds.CONCLUSIONS: This study revealed that the pigment PY3 from P.stutzeri has anticancer potential and induced cell death by apoptosis. It was found to have the carotenoid fucoxanthinol, responsible for its observed anticancer activity |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 4774 |
| Topical term or geographic name entry element | PHARMACOLOGY |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 11942 |
| Co-Author | Kilingar, Nadumane Varalakshmi |
| 773 0# - HOST ITEM ENTRY | |
| Place, publisher, and date of publication | Andheri - Mumbai Wolters Kluwer India Private Limited |
| Title | Indian Journal of Pharmacology |
| International Standard Serial Number | 0253-7613 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| URL | http://www.ijp-online.com/temp/IndianJPharmacol503116-2081508_054655.pdf |
| Link text | Click here |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Articles Abstract Database |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total Checkouts | Barcode | Date last seen | Price effective from | Koha item type |
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| Dewey Decimal Classification | School of Pharmacy | School of Pharmacy | Archieval Section | 06/02/2020 | 2020834 | 06/02/2020 | 06/02/2020 | Articles Abstract Database |