In silico Study, Protein Kinase Inhibition and Antiproliferative Potential of Flavonoids Isolated from Bassia eriophora (Schrad.) Growing in KSA (Record no. 15565)
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| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20211123145300.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 211123b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | AIKTC-KRRC |
| Transcribing agency | AIKTC-KRRC |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 14769 |
| Author | Arafa Musa |
| 245 ## - TITLE STATEMENT | |
| Title | In silico Study, Protein Kinase Inhibition and Antiproliferative Potential of Flavonoids Isolated from Bassia eriophora (Schrad.) Growing in KSA |
| 250 ## - EDITION STATEMENT | |
| Volume, Issue number | Vol.55(2), Apr-Jun |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Banaglore |
| Name of publisher, distributor, etc. | Association of Pharmaceutical Teachers of India (APTI) |
| Year | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Pagination | 483-490p. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Introduction: Kinase enzymes play an important role in cellular proliferation, the main target in cancer treatment is to inhibit their functions. Protein kinase inhibitors as flavonoids can be applied for prevention or treatment of cancers through inhibition of cell proliferation. Objectives: To isolate cytotoxic metabolites from B. eriophora, evaluate their antiproliferative and protein kinase inhibitory effects, as well as the in silico study for active compounds. Materials and Methods: Preparative HPLC was used for purification of the isolates. NMR, MS and UV spectroscopy were applied for characterization of the pure compounds. Sulphorhodamine-B and radiometric assay methods were employed for determination of the antiproliferative and protein kinase inhibition activities, respectively. The antiproliferative mechanism was predicted by in silico study using Molecular Operating Environment (MOE). Results: Five flavonoids; luteolin, acacetin-7-O-β-D-glucoside, diosmin, kaempferol-3-O-rutinoside and rutin were isolated and investigated for their antiproliferative and kinase inhibitory effects. Luteolin exhibited strong antiproliferative effect against certain cell lines including MCF-7, HepG2 and HCT-116 with IC50 (33.24 ± 0.83, 26.54 ±1.02 and 31.62 ±1.32 μM, respectively), while diosmin and kaempferol- 3-O-rutinoside showed strong antiproliferative effect against MCF-7 with IC50 (26.56 ± 1.12 and 28.72 ±0.98). Luteolin showed highest inhibitory effect against Aurora B and CDK4/CyclinD1 with IC50 3.16 and 4.95 m3.16 and 4.95 inhibit in slico study for the isolated metabolites against Aurora B and CDK4/CycD1 confirmed their cytotoxic profile. Conclusion: Five flavonoids were firstly isolated from B. eriophora. The putative antiproliferative mechanism of luteolin and kaempferol-3-O-rutinoside on Aurora B and CDK4/CycD1 kinases was predictable by in slico study. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 4639 |
| Topical term or geographic name entry element | PHARMACEUTICS |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 14770 |
| Co-Author | Al-Sanea, Mohammad Mahmoud |
| 773 0# - HOST ITEM ENTRY | |
| Title | Indian journal of pharmaceutical education and research |
| Place, publisher, and date of publication | Bengluru Association of Pharmaceutical Teachers of India (APTI) |
| International Standard Serial Number | 0019-5464 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| URL | https://www.ijper.org/sites/default/files/IndJPhaEdRes-55-2-483.pdf |
| Link text | Click here |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Articles Abstract Database |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total Checkouts | Barcode | Date last seen | Price effective from | Koha item type |
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| Dewey Decimal Classification | School of Pharmacy | School of Pharmacy | Archieval Section | 23/11/2021 | 2021-2022370 | 23/11/2021 | 23/11/2021 | Articles Abstract Database |