Lyophilized Fruit Juice of Citrus sinensis Triggers Mitochondria-mediated Apoptosis via Downregulation of PI3K/AKT in MCF-7 Cell Line (Record no. 15573)
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| fixed length control field | a |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20211124094534.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 211124b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | AIKTC-KRRC |
| Transcribing agency | AIKTC-KRRC |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 14783 |
| Author | Mathiyazhagan, Jayasindu |
| 245 ## - TITLE STATEMENT | |
| Title | Lyophilized Fruit Juice of Citrus sinensis Triggers Mitochondria-mediated Apoptosis via Downregulation of PI3K/AKT in MCF-7 Cell Line |
| 250 ## - EDITION STATEMENT | |
| Volume, Issue number | Vol.55(2), Apr-Jun |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Banaglore |
| Name of publisher, distributor, etc. | Association of Pharmaceutical Teachers of India (APTI) |
| Year | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Pagination | 556-565p. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Background: Breast cancer is prevalent cancer among women and a major cause of death worldwide due to various genetic and environmental factors. Citrus sinensis is widely taken fruit juice in most countries that are believed to reduce breast cancer risk. However, the mechanism through which it acts remains unclear. Our objective was to explore the mechanism through which Citrus sinensis acts as a cytotoxic agent. Materials and Methods: FT-IR and GC-MS analyses of lyophilzed orange juice (LOJ) were performed to find phytocompounds that were subjected to in silico docking analysis against phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway. The proteins that showed the low free energy of binding was taken for gene expression analysis. RT-PCR analysis was carried out to observe PI3K/AKT/mTOR genes inhibition in LOJ treated cells. Cytotoxicity, apoptosis, caspase 9 activity were performed against the human breast cancer cell line (MCF-7). Results: The docking analysis against PI3K/AKT/mTOR with LOJ compounds showed the best interactions. Further down-regulation of PI3K/AKT and moderate up-regulation of mTOR was confirmed using gene expression analysis. LOJ showed concentration-dependent cytotoxicity, apoptosis, decreased mitochondrial membrane stability and caspase 9 against the MCF-7 cell line. Conclusion: This study concludes that LOJ can induce apoptosis in MCF-7 cells via PI3K and AKT inhibition. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 4639 |
| Topical term or geographic name entry element | PHARMACEUTICS |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 14784 |
| Co-Author | Daniel, John Arul |
| 773 0# - HOST ITEM ENTRY | |
| International Standard Serial Number | 0019-5464 |
| Title | Indian journal of pharmaceutical education and research |
| Place, publisher, and date of publication | Bengluru Association of Pharmaceutical Teachers of India (APTI) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| URL | https://www.ijper.org/sites/default/files/IndJPhaEdRes-55-2-556.pdf |
| Link text | Click here |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Articles Abstract Database |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total Checkouts | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | School of Pharmacy | School of Pharmacy | Archieval Section | 24/11/2021 | 2021-2022378 | 24/11/2021 | 24/11/2021 | Articles Abstract Database |