Evaluation of the cytotoxicity, apoptosis and autophagy induction by fucoidan in human hepatic adenocarcinoma cells (Record no. 17334)
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| fixed length control field | a |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220826094207.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 220826b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | AIKTC-KRRC |
| Transcribing agency | AIKTC-KRRC |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 17609 |
| Author | Dubey, Akanksha |
| 245 ## - TITLE STATEMENT | |
| Title | Evaluation of the cytotoxicity, apoptosis and autophagy induction by fucoidan in human hepatic adenocarcinoma cells |
| 250 ## - EDITION STATEMENT | |
| Volume, Issue number | Vol.56(3), Jul-Sep |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Karnataka |
| Name of publisher, distributor, etc. | Association of Pharmaceutical Teachers of India (APTI) |
| Year | 2022 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Pagination | 756-764p. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Introduction: Seaweeds are the reservoir of bioactive constituents such as proteins,<br/>polysaccharides, alkaloids, steroids and terpenoids that possess antioxidant, anti-<br/>inflammatory and anti-cancerous properties.<br/>Dictyota bartayresiana is a brown seaweed<br/>with high antioxidant activity, making this species very valuable for human health.<br/>Fucoidan is the sulphated polysaccharide obtained from brown algae<br/>. Objectives: The<br/>study aimed to extract, purify and characterize fucoidan from<br/>Dictyota bartayresiana and<br/>investigate its antioxidant and anti-cancer properties. Materials and Methods: Fucoidan<br/>was isolated from<br/>Dictyota bartayresiana and was investigated for its antioxidant property<br/>by DPPH and ABTS assays, whereas cytotoxicity in SK-Hep-1 cells was checked using<br/>MTT assay. The apoptosis assay was achieved via the Annexin-V-Apoptosis detection<br/>kit, and images were obtained. To detect autophagy, western blotting was performed<br/>for protein Atg5. Results: Fucoidan revealed positive radical scavenging actions even at<br/>minor concentrations. The results acquired from the MTT assay revealed that cell death<br/>initiated at minor concentrations of 0.781μg/ml and was in a dose-dependent mode.<br/>The IC 50 value obtained was 30.96 (μg/ml). In our study, SK-Hep-1 cells revealed that<br/>the intensities of the emission bands altered in response to apoptosis. It was observed<br/>that after 24 hr of fucoidan treatment, the level of Atg5 increased with an increase in<br/>fucoidan concentration and the band size observed for Atg5 was 55kDa. Conclusion:<br/>Fucoidan possessed antioxidant and cytotoxic properties and was found helpful in<br/>managing hepatocellular carcinoma. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 4639 |
| Topical term or geographic name entry element | PHARMACEUTICS |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 17610 |
| Co-Author | Sivaraman, Jayanthi |
| 773 0# - HOST ITEM ENTRY | |
| Place, publisher, and date of publication | Bengluru Association of Pharmaceutical Teachers of India (APTI) |
| International Standard Serial Number | 0019-5464 |
| Title | Indian journal of pharmaceutical education and research |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| URL | https://www.ijper.org/sites/default/files/IndJPhaEdRes-56-3-756.pdf |
| Link text | Click here |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Articles Abstract Database |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total Checkouts | Barcode | Date last seen | Price effective from | Koha item type |
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| Dewey Decimal Classification | School of Pharmacy | School of Pharmacy | Archieval Section | 26/08/2022 | 2022-1385 | 26/08/2022 | 26/08/2022 | Articles Abstract Database |