Carthamus tinctorius l. Inhibits proliferation of lung cancer A549 cells and tender’s mitochondrial protection (Record no. 17338)
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| fixed length control field | a |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220826112820.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 220826b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | AIKTC-KRRC |
| Transcribing agency | AIKTC-KRRC |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 17617 |
| Author | Dailah, Hamad Ghaleb |
| 245 ## - TITLE STATEMENT | |
| Title | Carthamus tinctorius l. Inhibits proliferation of lung cancer A549 cells and tender’s mitochondrial protection |
| 250 ## - EDITION STATEMENT | |
| Volume, Issue number | Vol.56(3), Jul-Sep |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Karnataka |
| Name of publisher, distributor, etc. | Association of Pharmaceutical Teachers of India (APTI) |
| Year | 2022 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Pagination | 789-794p. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Background: Plant-based products are well-known as long-lasting chemo preventive and<br/>chemotherapeutic medicines against cancer. Objectives: The purpose of this research<br/>is to identify out how safflower extract affects A549 cells. Materials and Methods:<br/>The antiproliferative activity was determined using the Trypan blue assay, while the<br/>cytotoxicity was determined using the MTT assay. The ethidium bromide/acridine<br/>orange (AO/EB) dual staining method was used to observe the apoptotic inducing effect<br/>and the morphological assessment of A549 cells using phase contrast microscopy<br/>was studied to discern the nuclear changes if any on treatment with safflower.<br/>The mitochondrial membrane potential was studied using a cationic lipophilic dye<br/>rhodamine 123 under a confocal microscope, and oxidative stress was assessed using<br/>2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA) labelling. Results: The trypan blue<br/>assay speculated that there was a decrease in cell viability at highest concentration and<br/>that the effect was dose dependent. The MTT assay revealed that cytotoxicity increases<br/>in cells treated with higher concentration. The LC50 range of the sample was found at<br/>250 μg/ml concentration at 52.2%, whereas the viability of the cell declined to 28.19%<br/>at 350μg/ml concentration. The morphological features like shrinkage, detachment,<br/>membrane blebbing, and distorted shape were observed on safflower treated A549 cells<br/>that supports the antiproliferative activity. The ethidium bromide and acridine orange<br/>staining substantiates that safflower extract induces apoptosis wherein the untreated<br/>A549 cells showed green fluorescence with intact nuclear morphology and cells treated<br/>with safflower extract (200 μg and 250 μg/ml), showed apoptotic bodies, clearly<br/>validating the apoptosis inducing effect by safflower on A549 cells. Also, the safflower<br/>extract protects the mitochondria by decreasing the oxidative stress and by altering the<br/>mitochondrial membrane potential. Conclusion: Thus, it is found that safflower extract<br/>exerts its antiproliferative activity by inducing apoptosis and protects mitochondria by<br/>combating oxidative stress. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 4639 |
| Topical term or geographic name entry element | PHARMACEUTICS |
| 773 0# - HOST ITEM ENTRY | |
| Place, publisher, and date of publication | Bengluru Association of Pharmaceutical Teachers of India (APTI) |
| International Standard Serial Number | 0019-5464 |
| Title | Indian journal of pharmaceutical education and research |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| URL | https://www.ijper.org/sites/default/files/IndJPhaEdRes-56-3-789.pdf |
| Link text | Click here |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Articles Abstract Database |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total Checkouts | Barcode | Date last seen | Price effective from | Koha item type |
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| Dewey Decimal Classification | School of Pharmacy | School of Pharmacy | Archieval Section | 26/08/2022 | 2022-1389 | 26/08/2022 | 26/08/2022 | Articles Abstract Database |