In vitro acetylcholinesterase inhibitory activity of different capsicum varieties by using chicken brain extract prepared by employing a home mixer-jar as an alternative to expensive tissue homogenizer (Record no. 17341)
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| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220826121936.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 220826b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | AIKTC-KRRC |
| Transcribing agency | AIKTC-KRRC |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 17622 |
| Author | Pandy, Vijayapandi |
| 245 ## - TITLE STATEMENT | |
| Title | In vitro acetylcholinesterase inhibitory activity of different capsicum varieties by using chicken brain extract prepared by employing a home mixer-jar as an alternative to expensive tissue homogenizer |
| 250 ## - EDITION STATEMENT | |
| Volume, Issue number | Vol.56(3) Jul-Aug |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Karnataka |
| Name of publisher, distributor, etc. | Association of Pharmaceutical Teachers of India (APTI) |
| Year | 2022 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Pagination | 810-815p. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Objectives: The present study aimed to investigate acetylcholinesterase (AchE) inhibitory<br/>activity of methanolic extract of different capsicum varieties using isolated chicken’s<br/>brain homogenate as an alternative for rodent’s brain homogenate by Ellman’s<br/>in<br/>vitro assay. In this study, we used a home mixer-jar as a substitute for the expensive<br/>tissue homogenizer to mince the chicken brain along with conventional hand motor-<br/>pestle homogenization technique. Materials and Methods: This<br/>in vitro AchE inhibition<br/>assay was performed as described by Ellman<br/>et al. (1961) with minor modifications.<br/>Results: The IC 50 values in ng/ml of the reference drug, neostigmine were found to be<br/>149.92±37.72 and 347.22±81.50 using mortar and pestle (MP), and mixer-jar (MJ)<br/>method of tissue homogenization techniques, respectively. The IC50 values in μg/ml of the<br/>methanolic extract of different varieties of capsicum were found to be: red chilli by MP<br/>method 1786.1±118.52; by MJ method 1858.5 ±136.79, green chilli by MP method<br/>1418.8±94.16; by MJ method 1786.1±118.52and green capsicum (bell pepper) by<br/>MP method 2022.8±244.5; by MJ method1813.3 ±45.63, respectively. Furthermore,<br/>it was noted that the yield of AchE in brain extract prepared using a mixer jar was<br/>high when compared with the brain homogenate prepared using the mortar and pestle.<br/>Conclusion: The use of chicken brain and employing mixer-jar for brain homogenization<br/>are evolved as good alternatives for unnecessary killing of laboratory animals and for<br/>the brain tissue homogenization. All capsicum varieties (green chilli, red chilli, and green<br/>capsicum) showed AchE inhibitory effect in<br/>in vitro Ellman’s assay. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 4639 |
| Topical term or geographic name entry element | PHARMACEUTICS |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 17623 |
| Co-Author | Shaik, Baji Rahamtulla |
| 773 0# - HOST ITEM ENTRY | |
| Title | Indian journal of pharmaceutical education and research |
| Place, publisher, and date of publication | Bengluru Association of Pharmaceutical Teachers of India (APTI) |
| International Standard Serial Number | 0019-5464 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| URL | https://www.ijper.org/sites/default/files/IndJPhaEdRes-56-3-810.pdf |
| Link text | Click here |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Articles Abstract Database |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total Checkouts | Barcode | Date last seen | Price effective from | Koha item type |
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| Dewey Decimal Classification | School of Pharmacy | School of Pharmacy | Archieval Section | 26/08/2022 | 2022-1392 | 26/08/2022 | 26/08/2022 | Articles Abstract Database |