Antimicrobial, antioxidant and cytotoxic activities of different fractions obtained from endophyticfusarium solani F01 isolated in catharanthus roseus collected in vietnam (Record no. 17364)

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control field OSt
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control field 20220830093656.0
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fixed length control field 220830b xxu||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency AIKTC-KRRC
Transcribing agency AIKTC-KRRC
100 ## - MAIN ENTRY--PERSONAL NAME
9 (RLIN) 17664
Author Nguyen, Tu
245 ## - TITLE STATEMENT
Title Antimicrobial, antioxidant and cytotoxic activities of different fractions obtained from endophyticfusarium solani F01 isolated in catharanthus roseus collected in vietnam
250 ## - EDITION STATEMENT
Volume, Issue number Vol.56(2), Apr-June
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Karnataka
Name of publisher, distributor, etc. Association of Pharmaceutical Teachers of India (APTI)
Year 2022
300 ## - PHYSICAL DESCRIPTION
Pagination 461-469p.
520 ## - SUMMARY, ETC.
Summary, etc. Aim: Finding out the biological sources is essential for pharmaceutical field. Materials and<br/>Methods: Endophytic fungus was isolated from<br/>Catharanthus roseus originated in Vietnam<br/>and then identified by comparing, phylogeny analyzing its 18S sRNA sequence using<br/>Blast search and GenomeNet. The supernatant and sonicated mycelia were fractionated<br/>with methanol, ethyl acetate, hexane. All the fractions were used to test antimicrobial,<br/>antioxidant, cytotoxicity activities. Antimicrobial activities on<br/>Staphylococcus aureus<br/>ATCC 25923,<br/>Pseudomonas aeruginosa ATCC 27853,<br/>Serratia marcescens ATCC<br/>14756,<br/>Vibrio parahaemolyticus ATCC 17802, and<br/>Escherichia coli ATCC 25922 were<br/>performed by well diffusion method. In terms of antioxidant activities, reducing power<br/>and DPPH radical scavenging assay were done. The purification from methanol extract<br/>was performed. The purified compounds were used to test antioxidant activity. For<br/>cytotoxicity activity, sulforhodamine B assay was used to test the extracts and purified<br/>compounds on the breast cancer cell line (MCF-7). Results:<br/>Fusarium solani F01 was<br/>identified. The treated methanol extract from cell-free supernatant showed the highest<br/>activities and wider spectrum than ethyl acetate and hexane extracts. Mycelial extracts in<br/>these solvents did not show activities except ethyl acetate extract. The methanol crude<br/>extract from cell-free supernatant gave the higher reducing power (1.416 ± 0.067) than<br/>ascorbic acid (0.577 ± 0.01) while others were very low at the concentration (100 μg/<br/>ml). Only methanol extract obtained from supernatant showed moderate scavenging<br/>activity when comparison of their IC50 (9.17 g/ml) with IC50 of ascorbic acid (1.28 g/<br/>ml). Four compounds were obtained that showed antioxidant activities, but there was<br/>one purified compound showed low effects on MCF-7 while methanol extract gave the<br/>highest inhibition (80.34 ± 1.28). Conclusion: The study suggested fungus isolated<br/>from<br/>Catharanthus roseus could show and give many biological compounds that will be<br/>a potential source for pharmaceutical field.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 4639
Topical term or geographic name entry element PHARMACEUTICS
700 ## - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 17665
Co-Author Doan, Vinh
773 0# - HOST ITEM ENTRY
Title Indian journal of pharmaceutical education and research
Place, publisher, and date of publication Bengluru Association of Pharmaceutical Teachers of India (APTI)
International Standard Serial Number 0019-5464
856 ## - ELECTRONIC LOCATION AND ACCESS
URL https://www.ijper.org/sites/default/files/IndJPhaEdRes-56-2-461.pdf
Link text Click here
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    Dewey Decimal Classification     School of Pharmacy School of Pharmacy Archieval Section 30/08/2022   2022-1431 30/08/2022 30/08/2022 Articles Abstract Database
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