Development of fast, precise and selective RP-HPLC methods for identification of possible degradation products of ivermectin in isolated rat hepatocytes and in different pH media (Record no. 17374)

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fixed length control field a
003 - CONTROL NUMBER IDENTIFIER
control field OSt
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20220901094751.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 220901b xxu||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency AIKTC-KRRC
Transcribing agency AIKTC-KRRC
100 ## - MAIN ENTRY--PERSONAL NAME
9 (RLIN) 17680
Author Dineva, Alexandrina
245 ## - TITLE STATEMENT
Title Development of fast, precise and selective RP-HPLC methods for identification of possible degradation products of ivermectin in isolated rat hepatocytes and in different pH media
250 ## - EDITION STATEMENT
Volume, Issue number Vol.56(2), Apr-June
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Karnataka
Name of publisher, distributor, etc. Association of Pharmaceutical Teachers of India (APTI)
Year 2022
300 ## - PHYSICAL DESCRIPTION
Pagination 546-552p.
520 ## - SUMMARY, ETC.
Summary, etc. Aim: A new stability indicating RP-HPLC based methods were developed to identify the<br/>possible degradation products of Ivermectin in isolated rat hepatocytes and in different<br/>pH media using a C18 RP column. Complete validation, including linearity, accuracy,<br/>recovery, precision, robustness, stability, and peak purity, was performed. Materials<br/>and Methods: HPLC system of UltiMateDionex 3000 DAD with LiChrosorb C18 Column<br/>were used in this study. During the investigation, for the removal of interfering fraction<br/>of isolated rat hepatocytes a purification procedure, consisting of protein precipitation<br/>followed by double filtration was developed. Results: Method I was applied effectively<br/>and the results indicated two major hepatic metabolites of Ivermectin. Method II was<br/>developed to monitor the chemical stability in close to physiological conditions. The<br/>appearance of a new peak and the proportional decrease in the sample concentration<br/>indicate a liability in buffer with pH12. Conclusion: The obtained results demonstrated<br/>that the degradation products of Ivermectin in hepatic cells and in alkali media are<br/>different.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 4639
Topical term or geographic name entry element PHARMACEUTICS
700 ## - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 17681
Co-Author Angelov, Borislav
773 0# - HOST ITEM ENTRY
International Standard Serial Number 0019-5464
Place, publisher, and date of publication Bengluru Association of Pharmaceutical Teachers of India (APTI)
Title Indian journal of pharmaceutical education and research
856 ## - ELECTRONIC LOCATION AND ACCESS
URL https://www.ijper.org/sites/default/files/IndJPhaEdRes-56-2-546.pdf
Link text Click here
942 ## - ADDED ENTRY ELEMENTS (KOHA)
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Koha item type Articles Abstract Database
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    Dewey Decimal Classification     School of Pharmacy School of Pharmacy Archieval Section 01/09/2022   2022-1447 01/09/2022 01/09/2022 Articles Abstract Database
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