Rapid simultaneous quantitative analysis of hypoglycemic agents by RP HPLC: development, validation and application to medicine (Record no. 17376)

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003 - CONTROL NUMBER IDENTIFIER
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005 - DATE AND TIME OF LATEST TRANSACTION
control field 20220901103813.0
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fixed length control field 220901b xxu||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency AIKTC-KRRC
Transcribing agency AIKTC-KRRC
100 ## - MAIN ENTRY--PERSONAL NAME
9 (RLIN) 10005
Author Attimarad, Mahesh
245 ## - TITLE STATEMENT
Title Rapid simultaneous quantitative analysis of hypoglycemic agents by RP HPLC: development, validation and application to medicine
250 ## - EDITION STATEMENT
Volume, Issue number Vol.56(2), Apr-June
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Karnataka
Name of publisher, distributor, etc. Association of Pharmaceutical Teachers of India (APTI)
Year 2022
300 ## - PHYSICAL DESCRIPTION
Pagination 564-572p.
520 ## - SUMMARY, ETC.
Summary, etc. Background: For the treatment of diabetes mellitus type 2, a new formulation containing<br/>vildagliptin and remogliflozin was developed. A simple and rapid RP-HPLC method<br/>employing linagliptin as an internal standard was developed for quality control of this<br/>medicine. Methodology: Formulation analytes, including IS, were separated on a Zorbax<br/>C18 column with isocratic elution of acetonitrile and phosphate buffer (pH 5) 55:45 v/v<br/>at a flow rate of 1.2 mL/min. The experiment was carried out at room temperature and<br/>monitored at a wavelength of 210 nm. The approach was also validated in accordance<br/>with the ICH Q2 requirements. Results: The optimized HPLC approach revealed a<br/>satisfactory linearity in the concentration ranges of 10-60 μg/mL and 10-100 μg/mL for<br/>VIL and REM respectively, with good regression coefficient (<br/>R2≥0.998). The average<br/>accuracy for VL and REM was 99.57 percent and 100.59 percent, respectively, with a<br/>low percentage relative error. The method’s precision was proven by the low percentage<br/>relative standard deviation. Furthermore, a robustness assessment employing a Pareto<br/>chart generated using a three-level factor interaction study, a multivariate technique,<br/>demonstrated that minor changes in individual experimental conditions had no effect<br/>on the test results. Finally, the optimized HPLC method was effectively used to assess<br/>VIL and Rem from formulation simultaneously. Conclusion: The findings of an assay<br/>comparing a simple and rapid isocratic RP-HPLC method devised for the simultaneous<br/>quantification of VIL and REM to a previously published approach revealed no significant<br/>differences in the assay results. As a result, it might be utilized in any analytical laboratory<br/>for quality control of this formulation.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 4639
Topical term or geographic name entry element PHARMACEUTICS
700 ## - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 17684
Co-Author Venugopala, Katharigatta Narayanaswamy
773 0# - HOST ITEM ENTRY
Title Indian journal of pharmaceutical education and research
International Standard Serial Number 0019-5464
Place, publisher, and date of publication Bengluru Association of Pharmaceutical Teachers of India (APTI)
856 ## - ELECTRONIC LOCATION AND ACCESS
URL https://www.ijper.org/sites/default/files/IndJPhaEdRes-56-2-564.pdf
Link text Click here
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    Dewey Decimal Classification     School of Pharmacy School of Pharmacy Archieval Section 01/09/2022   2022-1449 01/09/2022 01/09/2022 Articles Abstract Database
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