Evaluation of cell death potential of lepidium sativum seed extracts in MCF-7 cells and molecular docking-based correlation of identified bioactive components with human caspase-6 protein (Record no. 17616)
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| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220922162459.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 220922b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | AIKTC-KRRC |
| Transcribing agency | AIKTC-KRRC |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 18056 |
| Author | Rajasekaran, R. |
| 245 ## - TITLE STATEMENT | |
| Title | Evaluation of cell death potential of lepidium sativum seed extracts in MCF-7 cells and molecular docking-based correlation of identified bioactive components with human caspase-6 protein |
| 250 ## - EDITION STATEMENT | |
| Volume, Issue number | Vol.56(1), Jan-Mar |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Karnataka |
| Name of publisher, distributor, etc. | Association of Pharmaceutical Teachers of India (APTI) |
| Year | 2022 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Pagination | 166-174p. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Aim and Background: The methanolic extract of<br/>Lepidium sativum L. was known for its<br/>free radical scavenging potential and anticancer properties. The aim was to perform a<br/>comparative investigation of the cytotoxic and cell death potential of the Soxhlet (SOX)<br/>and crude methanolic extract (CRU). `Materials and Methods: MTT as well as the<br/>PI-based assays in caspase-3-deficient, MCF-7 human breast cancer cells, for its cytotoxic<br/>potential and synergistic effect. LC-QTOF-MS/MS was used for characterization. These<br/>bioactive molecules were docked with human caspase-6 (2WDP) as well as its zymogen<br/>variant (4IYR) to mimicking a more physiological form of cell demise. Results: Both<br/>extracts showed a dose-dependent toxicity and the IC50 was found to be 136.75μg/ml,<br/>and 88.49 μg/ml compared with that of standard quercetin (8.72μg/ml). At their IC50<br/>concentrations, the corresponding PI-based flow cytometry and cell death values were<br/>31.5±3% and 32.4±5.6, while that of quercetin was 36.9±7.4 %. A synergy in cell<br/>death was seen for a combination of<br/>L. sativum extracts (CRU-MeOH & SOX-MeOH)<br/>and quercetin. Thirteen (13) compounds were reported for the first time in this seed by<br/>LC-MS/MS. Good binding affinity was seen with both human caspase -6 (2WDP) and<br/>the mutated zymogen (4IYR) with reference to that of quercetin. Conclusion: This<br/>in vitro/<br/>in silico correlation showed that the methanolic extracts of<br/>L. sativum exhibited a<br/>significantly higher level of cell death in MCF-7 cells. Also, their synergistic increases in<br/>cell death provides a basis possibly for a combination therapy-based strategy. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 4774 |
| Topical term or geographic name entry element | PHARMACOLOGY |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 18057 |
| Co-Author | Suresh P. K. |
| 773 0# - HOST ITEM ENTRY | |
| International Standard Serial Number | 0019-5464 |
| Title | Indian journal of pharmaceutical education and research |
| Place, publisher, and date of publication | Bengluru Association of Pharmaceutical Teachers of India (APTI) |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| URL | https://www.ijper.org/sites/default/files/IndJPhaEdRes-56-1-166.pdf |
| Link text | Click here |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Articles Abstract Database |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total Checkouts | Barcode | Date last seen | Price effective from | Koha item type |
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| Dewey Decimal Classification | School of Pharmacy | School of Pharmacy | Archieval Section | 22/09/2022 | 2022-1712 | 22/09/2022 | 22/09/2022 | Articles Abstract Database |