Development and validation of RP-HPLC method for simultaneous qualitative and quantitative estimation of curcumin and quercetin in bulk mixture (Record no. 17633)
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| 000 -LEADER | |
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| fixed length control field | a |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220923151027.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 220923b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | AIKTC-KRRC |
| Transcribing agency | AIKTC-KRRC |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 18082 |
| Author | Patil, Nayana |
| 245 ## - TITLE STATEMENT | |
| Title | Development and validation of RP-HPLC method for simultaneous qualitative and quantitative estimation of curcumin and quercetin in bulk mixture |
| 250 ## - EDITION STATEMENT | |
| Volume, Issue number | Vol.56(1), Jan-Mar |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Karnataka |
| Name of publisher, distributor, etc. | Association of Pharmaceutical Teachers of India (APTI) |
| Year | 2022 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Pagination | 247-254p. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Objectives: To design a definite, new and explicit reverse phase high performance liquid<br/>chromatographic (RP-HPLC) method for simultaneous estimation of quantitative and<br/>qualitative curcumin and quercetin in bulk mixture. As per guidelines of International<br/>conference on harmonization (ICH) method was validated. Materials and Methods: In this<br/>study, reverse phase analysis was used to do the analysis Younglin- ACME 9000, C18<br/>(250 x 4.6mm i.d) solvent system methanol and 0.05% orthophosphoric acid as mobile<br/>phase in proportion of 80:20 v/v was used. The flow rate at constant rate 0.7ml/min.<br/>The absorbance of the eluted sample was analyzed by use of UV Visible detector at<br/>254 nm. Results: The proposed improved methodology takes 5.25 min to elute curcumin<br/>and 8.78 min to elute quercetin. The system suitability parameters were studied for the<br/>developed method and were found to be within the acceptable limits. The LOQ and LOD<br/>of curcumin was reported to be 0.041 and 0.081g/ml, respectively, while quercetins<br/>were 0.0048 and 0.039g/ml. Conclusion: The designed methodology validated as per the<br/>ICH guidelines for its linearity, precision, specificity, sensitivity, accuracy, raggedness,<br/>robustness, LOD, and LOQ. The results of the preceding observations show that the<br/>procedure is effective in both qualitative and quantitative analyses of the markers in the mixture. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 4639 |
| Topical term or geographic name entry element | PHARMACEUTICS |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 18083 |
| Co-Author | Mahajan, Hitendra |
| 773 0# - HOST ITEM ENTRY | |
| Place, publisher, and date of publication | Bengluru Association of Pharmaceutical Teachers of India (APTI) |
| Title | Indian journal of pharmaceutical education and research |
| International Standard Serial Number | 0019-5464 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| URL | https://www.ijper.org/sites/default/files/IndJPhaEdRes-56-1-247.pdf |
| Link text | Click here |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Articles Abstract Database |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total Checkouts | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | School of Pharmacy | School of Pharmacy | Archieval Section | 23/09/2022 | 2022-1723 | 23/09/2022 | 23/09/2022 | Articles Abstract Database |