Development and validation of a precise RP- HPLC method to determine gentiopicroside content in cultures of gentiana kurroo royle (Record no. 17636)
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| 000 -LEADER | |
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| fixed length control field | a |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220923152454.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 220923b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | AIKTC-KRRC |
| Transcribing agency | AIKTC-KRRC |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 18087 |
| Author | Alphonse, Mariadoss |
| 245 ## - TITLE STATEMENT | |
| Title | Development and validation of a precise RP- HPLC method to determine gentiopicroside content in cultures of gentiana kurroo royle |
| 250 ## - EDITION STATEMENT | |
| Volume, Issue number | Vol.56(1), Jan-Mar |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Karnataka |
| Name of publisher, distributor, etc. | Association of Pharmaceutical Teachers of India (APTI) |
| Year | 2022 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Pagination | 264-271p. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Background: Gentipicroside (GPD) is a major bioactive seco-iridoid glycoside in the<br/>methanolic extracts of roots and rhizomes of<br/>Gentiana kurroo Royle. GPD has anti-<br/>inflammatory, antidiabetic, analgesic, antinociceptive, antibacterial and free radical<br/>scavenging activities. Although this compound was analyzed by various methods in<br/>different<br/>Gentiana species previously, no valid method was documented describing<br/>the accuracy and precision for the detection and quantification of GPD from<br/>in vitro<br/>samples of<br/>G. kurroo. Materials and Methods: A simple, accurate and highly sensitive<br/>reversed-phase high-performance liquid chromatographic (RP-HPLC) method was<br/>developed and validated according to the International Conference on Harmonization<br/>(ICH) guidelines. Results: Methanol and 0.1% acetic acid in millipore water [30:70, (v/v)]<br/>was used as a mobile phase and eluted at an isocratic flow rate of 1.0 ml/min under<br/>room temperature. The calibration curve was linear in the concentration range of 10-100<br/>μg/ml with a correlation coefficient of 0.9967. The relative standard deviation (RSD)<br/>of repeatability and inter-day precision was less than 2%. The limit of detection (LOD)<br/>and limit of quantification (LOQ) of GPD was 0.083 μg/ml and 0.25 μg/ml, respectively.<br/>Recoveries from<br/>in vitro samples ranged from 91.0 to 114.0% and the precision of the<br/>method in terms of retention time (%RSD ≤ 2.01) and peak area (% RSD ≤5.11) were<br/>satisfactory. Conclusion: The validated RP-HPLC- PDA method can be used routinely for<br/>the determination of GPD in<br/>in vitro cultures and in vivo plants of<br/>G. kurroo. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 4639 |
| Topical term or geographic name entry element | PHARMACEUTICS |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 18088 |
| Co-Author | Chandrasekaran, Rajasekaran |
| 773 0# - HOST ITEM ENTRY | |
| Title | Indian journal of pharmaceutical education and research |
| Place, publisher, and date of publication | Bengluru Association of Pharmaceutical Teachers of India (APTI) |
| International Standard Serial Number | 0019-5464 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| URL | https://www.ijper.org/sites/default/files/IndJPhaEdRes-56-1-264.pdf |
| Link text | Click here |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Koha item type | Articles Abstract Database |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Home library | Current library | Shelving location | Date acquired | Total Checkouts | Barcode | Date last seen | Price effective from | Koha item type |
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| Dewey Decimal Classification | School of Pharmacy | School of Pharmacy | Archieval Section | 23/09/2022 | 2022-1725 | 23/09/2022 | 23/09/2022 | Articles Abstract Database |