Detection of novel candidate mutations as a cause of steroid-resistant nephrotic syndrome in children using next-generation sequencing techniques (Record no. 18668)

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control field OSt
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20230113093423.0
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fixed length control field 230113b xxu||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency AIKTC-KRRC
Transcribing agency AIKTC-KRRC
100 ## - MAIN ENTRY--PERSONAL NAME
9 (RLIN) 19687
Author Alsanie, Walaa F.
245 ## - TITLE STATEMENT
Title Detection of novel candidate mutations as a cause of steroid-resistant nephrotic syndrome in children using next-generation sequencing techniques
250 ## - EDITION STATEMENT
Volume, Issue number Vol.56(4), Oct_Dec
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Bangalore
Name of publisher, distributor, etc. Association of Pharmaceutical Teachers of India (APTI)
Year 2022
300 ## - PHYSICAL DESCRIPTION
Pagination 1134-1140p.
520 ## - SUMMARY, ETC.
Summary, etc. Background and Objectives: Steroid-resistant nephrotic syndrome (SRNS) is a serious<br/>chronic ailment that affects children and causes blood coagulation issues as well as an<br/>increased vulnerability to infections. Only around 10% of inherited genetic nephrotic<br/>syndrome cases are responding to steroid therapy, and, accordingly, 90% of SRNS<br/>patients have multidrug resistance. This study was done to detect novel candidate<br/>mutations as a factor for causing SRNS in children using the sequencing technique.<br/>Materials and Methods: This study included nine children ranging in age from one to<br/>sixteen years old who had a clinical diagnosis of SRNS. Phenotype-genotype correlations<br/>in these Saudi children were explored using next-generation sequencing techniques<br/>to assess the correlation and/or effect of mutations in multiple genes on phenotype<br/>variability. The enrichment analysis was carried out to identify genes. Results: Five<br/>genes were potentially new causative agents for SRNS. The enrichment analysis helped<br/>us identify nine causal genes, not previously reported, in six out of nine individuals<br/>(66%). These genes are phospholipase D family member 3, mitogen-activated protein<br/>kinase binding protein 1, solute carrier family 12 members 3, ezrin, and pancreatic lipase<br/>related protein. The other four nominee genes were wilms tumor 1, diacylglycerol kinase<br/>iota, coenzyme Q8B, and CASC3. Conclusion: The outcome of the study indicated that<br/>there is a new mutation as we had four replicates for each sample run on a different<br/>sequencing lane. The histopathological findings of these mutated patients were focal<br/>segmental glomerulosclerosis.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 4639
Topical term or geographic name entry element PHARMACEUTICS
700 ## - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 14735
Co-Author Alamri, Abdulhakeem S.
773 0# - HOST ITEM ENTRY
Place, publisher, and date of publication Bengluru Association of Pharmaceutical Teachers of India (APTI)
Title Indian journal of pharmaceutical education and research
International Standard Serial Number 0019-5464
856 ## - ELECTRONIC LOCATION AND ACCESS
URL https://www.ijper.org/sites/default/files/IJPER-Basheeruddin%20Asdaq_QCRD.pdf
Link text Click here
942 ## - ADDED ENTRY ELEMENTS (KOHA)
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    Dewey Decimal Classification     School of Pharmacy School of Pharmacy Archieval Section 13/01/2023   2023-0111 13/01/2023 13/01/2023 Articles Abstract Database
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