Effects of Ginkgo biloba extract on the apoptosis of oxygen and glucose-deprived SH-SY5Y cells and its mechanism (Record no. 23704)

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fixed length control field 251203b |||||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency AIKTC-KRRC
Transcribing agency AIKTC-KRRC
100 ## - MAIN ENTRY--PERSONAL NAME
Author Ba, Xiao-Hong
9 (RLIN) 27655
245 ## - TITLE STATEMENT
Title Effects of Ginkgo biloba extract on the apoptosis of oxygen and glucose-deprived SH-SY5Y cells and its mechanism
250 ## - EDITION STATEMENT
Volume, Issue number Vol.47(1), Jan-Feb
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Mumbai
Name of publisher, distributor, etc. Wolter Kluwer
Year 2015
300 ## - PHYSICAL DESCRIPTION
Pagination 101-104p.
520 ## - SUMMARY, ETC.
Summary, etc. <br/>Objective: <br/><br/>The aim was to observe the effects of the extract of Ginkgo biloba (EGb761) on the apoptosis of oxygen and glucose-deprived (OGD) human neuroblastoma cells (SH-SY5Y) cells and explore its mechanism.<br/>Materials and Methods: <br/><br/>SH-SY5Y cells were divided into normal control group, OGD group, OGD for 4 h and EGb761-pretreated groups including very low-concentration (20 μg/ml), low-concentration group (25 μg/ml), moderate-concentration group (50 μg/ml) and high-concentration group (100 μg/ml). Twenty four hours after reoxygenation, cell viability was determined with 3-[4, 5-dimehyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium bromide assay, apoptosis rate was detected with annexin V-fluorescein isothiocyanate/propidium iodide double staining flow cytometry and the protein level of apoptosis-inducing factor (AIF) was observed with immunofluorescence technique in each group.<br/>Results: <br/><br/>Cell viability was significantly lower in OGD group than in EGb761-pretreated groups, especially in moderate-concentration group (50 μg/ml) (P < 0.005). Apoptosis rate was significantly lower in EGb761-pretreated groups than in OGD group (P < 0.001). Immunofluorescent staining showed that there was AIF nuclear translocation in both EGb761-pretreated groups and OGD group, but AIF nuclear translocation was less in EGb761-pretreated groups than in OGD group.<br/>Conclusion: <br/><br/>EGb761 can reduce the apoptosis of OGD SH-SY5Y cells probably through inhibiting AIF nuclear translocation. This study provides a theoretical basis for the application of EGb761 in clinical practice.<br/>
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name entry element PHARMACOLOGY
9 (RLIN) 4774
700 ## - ADDED ENTRY--PERSONAL NAME
Co-Author Min, Lian-Qiu
9 (RLIN) 27656
773 0# - HOST ITEM ENTRY
Place, publisher, and date of publication Andheri - Mumbai Wolters Kluwer India Private Limited
International Standard Serial Number 0253-7613
Title Indian Journal of Pharmacology
856 ## - ELECTRONIC LOCATION AND ACCESS
URL https://journals.lww.com/iphr/fulltext/2015/47010/effects_of_ginkgo_biloba_extract_on_the_apoptosis.19.aspx
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    Dewey Decimal Classification     School of Pharmacy School of Pharmacy Archieval Section 03/12/2025   2025-1742 03/12/2025 03/12/2025 Articles Abstract Database
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