Development of a simple and sensititive HPLC method for the determination of rifaximin in serum and its application (Record no. 8498)

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fixed length control field a
003 - CONTROL NUMBER IDENTIFIER
control field OSt
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20190315101324.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 190312b xxu||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency AIKTC-KRRC
Transcribing agency AIKTC-KRRC
100 ## - MAIN ENTRY--PERSONAL NAME
9 (RLIN) 7994
Author Hossain, M. A.
245 ## - TITLE STATEMENT
Title Development of a simple and sensititive HPLC method for the determination of rifaximin in serum and its application
250 ## - EDITION STATEMENT
Volume, Issue number Vol. 80(6), November-December
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Mumbai
Year 2018
Name of publisher, distributor, etc. Indian Journal of Pharmaceutical Science
300 ## - PHYSICAL DESCRIPTION
Pagination 1108-1114
520 ## - SUMMARY, ETC.
Summary, etc. Rifaximin, a virtually non-absorbed rifamycin drug is gaining attention for its broad-spectrum of activity. A couple of methods have been reported for its determination in biological matrices. However, inconvenient sample preparation procedure, lack of sensitivity and long analysis time hindered the application of those methods in pharmacokinetic and toxicological study. Thus, a high performance liquid chromatography method has been developed and validated in this study for the sensitive quantification of rifaximin in serum. In this procedure, chromatographic separation was achieved by flowing a mobile phase comprise of acetonitrile and 0.1 % acetic acid (60:40, v/v) through a C18 column (150×4.6 mm, 5 μm). An ultraviolet detector was utilized to identify and quantify the compound. The method has been validated over a concentration range of 0.03-30.00 μg/ml (r2=0.9999) by utilizing 150 μl of serum. The accuracy of the method was 89.59-98.84 %, and the intra- and inter-day precisions were 0.41-6.84 and 1.83-5.71 %, respectively. The compound was stable in different storage conditions. Insignificant amounts (2.40±0.38 to 4.22±1.10 μg/ml) of rifaximin were quantified in serum samples of rat from 2 to 8 h after oral administration. This method offers a rapid, sensitive, specific, reproducible, and stable tool for the quantitative determination of rifaximin in serum, which could be applicable in other biological matrices and species for pharmacokinetic and toxicological study of rifaximin.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 4639
Topical term or geographic name entry element PHARMACEUTICS
700 ## - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 7995
Co-Author Pervin, Rokeya
700 ## - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 7996
Co-Author Park, Na-Hye
773 0# - HOST ITEM ENTRY
International Standard Serial Number 0250-474X
Title Indian journal of pharmaceutical sciences
Place, publisher, and date of publication New Delhi Indian Pharmaceutical Association
856 ## - ELECTRONIC LOCATION AND ACCESS
URL http://www.ijpsonline.com/articles/development-of-a-simple-and-sensitive-hplc-method-for-the-determination-of-rifaximin-in-serum-and-its-application-3570.html
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    Dewey Decimal Classification     School of Pharmacy School of Pharmacy Archieval Section 30/03/2019   2018450 19/06/2019 30/03/2019 Articles Abstract Database
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