Concrete is a reliable construction material in resisting fire. But, when exposed to high temperature for longer duration, it starts losing its structural performance. Nowadays, researches are being carried out on bacterial concrete. Bacteria produces CaCO3 as a result of metabolism, which seals the pores and improves strength of concrete. In this study, an attempt has been made to investigate the fire performance of Quaternary Blended Bacterial Self-Compacting Concrete (QBBSCC), a blend of 40% cement, 10% microsilica, 25% flyash and 25% ground granulated blast furnace slag (GGBFS). Bacillus Subtilis was used. For water- binder (w/b) ratios of 0.3 and 0.4, super-plasticiser of 1.8% and 1.6% by weight of binder, respectively, were used. QBBSCC cubes were subjected to temperatures of 200ºC, 400ºC, 600ºC, 800ºC, and 1000ºC for durations of 4, 8 and 12 hours. Weight loss and residual compressive strength were found. QBBSCC exhibited better temperature resistance than reference concrete without bacteria (QBSCC).