Objective:
In the domain of nano drug delivery, dendrimers are the most explored bioactive polymeric carrier system. The present work was aimed
to study the
diffusion potential of different generations of Poly
(propyleneimine
) (PPI) dendrimers
on goat nasal mucosa in an
ex vivo
study and
synthesize a stable dendrimer for olfactory drug delivery.
Method
s:
The generations (3.0G, 4.0G, and 5.0G) of PPI dendrimer were synthesized, and PEGylated by MPEG 5000 and then loaded with donepezil.
A comparative study was
carried out among all generations in term of their drug loading capacity, stability, sustained release
behaviour
as well as
for targeting efficacy. An
ex
-
vivo
study
was
carried out on Franz Diffusion Cell with goat nasal mucosa.
Result
s:
The
developed G3, G4, and G5
dendrimer
formulations
had entrapment efficiency of 24.33
±0.56%, 40.12
±0.62%, and 60.4
±0.6%,
respectively.
The n
asal diffusion study revealed that
5.0G PPI dendrimer increased
diffusion of donepezil up to 47% as compared to the pure
solution of donepezil while 10% improvement in diffusion
was seen
as compared to 4.0 G PPI dendrimer.
Thus obtained results claimed that the
drug loading as well as targeting potential of PPI dendrimers increase
d with the increase
in the number
of generation.
The investigation outcome
indicated promising results of 5.0G PPI dendrimer over the 3.0G and 4.0G PPI dendrimer generations for their drug loading cap
acity, sta
bility, and
sustained release action.