Standardization and application of pcr targeting chlorella species isolated from environmental samples
Publication details: M P Innovare Academic Sciences Pvt Ltd 2021Edition: Vol.13(7)Description: 28-31pSubject(s): Online resources: In: International journal of pharmacy and pharmaceutical scienceSummary: Objective: Identification of Chlorella species from the environment through 18s ribosomal RNA sequencing. This study was aimed to design primer targeting Chlorella and other closely related algal species targeting 18s ribosomal RNA, ITS1 region.Methods: Sanger sequencing was carried out for the identification of algae up to the genus and species level using an in-house designed primer and optimized PCR conditions.Results: Out of 2 algae samples identified phenotypically, one isolate identified as Chlorellavulgaris and other one identified as Chlorellasorokinianabased on the results of Basic Alignment Search Tool (BLAST).Conclusion: To conclude, this study provided primers with PCR conditions to characterize algal samples through molecular identification with 100% accuracy than the phenotypic method.| Item type | Current library | Status | Barcode | |
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School of Pharmacy Archieval Section | Not for loan | 2021-2022527 |
Objective: Identification of Chlorella species from the environment through 18s ribosomal RNA sequencing. This study was aimed to design primer targeting Chlorella and other closely related algal species targeting 18s ribosomal RNA, ITS1 region.Methods: Sanger sequencing was carried out for the identification of algae up to the genus and species level using an in-house designed primer and optimized PCR conditions.Results: Out of 2 algae samples identified phenotypically, one isolate identified as Chlorellavulgaris and other one identified as Chlorellasorokinianabased on the results of Basic Alignment Search Tool (BLAST).Conclusion: To conclude, this study provided primers with PCR conditions to characterize algal samples through molecular identification with 100% accuracy than the phenotypic method.
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