Production and identification of intricate bioactive oligosaccharides from Nyctanthesarbor-tristis leaves by a combination of enzymatic, HPAEC and MALDI-TOF-MS techniques
Publication details: New Delhi CSIR 2021Edition: Vol.60(B), NovDescription: 1471-1477pSubject(s): Online resources: In: Indian journal of chemistry (Section B)Summary: Non-digestible oligosaccharides are associated with positive health outcomes; however, hemicellulosic oligosaccharides of N. arbor-tristis have not been studied. Herein, we report production of hemicellulosic oligosaccharides from its leaves and their identification. A series of complex neutral oligosaccharides ( 1-16), produced by endo-(1,4)--D-glucanase digestion of the alkali extracted xyloglucan, have been identified by chemical, HPAE chromatographic and MALDI-mass spectrometric techniques. Additionally, xylan enriched hemicellulose-A fraction upon endo-(1,4)--D-xylanase digestion yield several acidic and neutral xylo-oligosaccharides ( 17-24), each containing a backbone of -(1,4)-D-Xylp units substituted at position 2 with a single stub of D-Xylp or 4-O-Me-D-GlcpA residues. The structural diversity and high water solubility of these enzyme-generated oligosaccharides suggested promising health implications.| Item type | Current library | Status | Barcode | |
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School of Pharmacy Archieval Section | Not for loan | 2022-0171 |
Non-digestible oligosaccharides are associated with positive health outcomes; however, hemicellulosic oligosaccharides
of N. arbor-tristis have not been studied. Herein, we report production of hemicellulosic oligosaccharides from its leaves
and their identification. A series of complex neutral oligosaccharides ( 1-16), produced by endo-(1,4)--D-glucanase
digestion of the alkali extracted xyloglucan, have been identified by chemical, HPAE chromatographic and MALDI-mass
spectrometric techniques. Additionally, xylan enriched hemicellulose-A fraction upon endo-(1,4)--D-xylanase digestion
yield several acidic and neutral xylo-oligosaccharides ( 17-24), each containing a backbone of -(1,4)-D-Xylp units
substituted at position 2 with a single stub of D-Xylp or 4-O-Me-D-GlcpA residues. The structural diversity and high water
solubility of these enzyme-generated oligosaccharides suggested promising health implications.
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