Background: Colorectal cancer (CRC) has grown to be the world’s fourth-biggest cause
of cancer-related mortality. It is critical to identify more effective treatment options for
it. Objectives: Doxorubicin a potent antineoplastic agent is widely used but has severe
adverse effects. Therefore, our objective is to use cubosome as an efficient drug delivery
system to reduce the off-target effects and increase the cytotoxic effects in CRC cells.
Materials and Methods: Pluronic F127-Based Cubosomes were prepared by low energy
stirring method and loaded with doxorubicin. Physicochemical characteristics were
studied using X-ray diffraction, dynamic light scattering, microscopic techniques, FTIR
UV spectrophotometer, and entrapment efficiency.
In vitro activity of doxorubicin-loaded
cubosomes was studied on HCT-116 cells. Results: The prepared cubosomes have the
required nano-size and the FTIR results showed the presence of both doxorubicin and
Pluronic F-127. The entrapment efficiency of doxorubicin was high too. DOX-CBs have
a better cytotoxic effect and induced ROS and reduced NO levels in HCT-116 cells.
Conclusion: The obtained results show that doxorubicin-loaded cubosomes can be used
to increase the cytotoxic effect in CRC cells and can be used with other chemotherapeutic
agents.