Identification of antioxidant and antidiabetic compounds in hedychium spicatum using TLC bioautography coupled with mass spectrometry and their in silico molecular docking studies
Publication details: Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022Edition: Vol.56(3), Jul-SepDescription: 830-837pSubject(s): Online resources: In: Indian journal of pharmaceutical education and researchSummary: Background: Traditional system of medicine has been practised since a long time to cure various diseases. More than 60-80 percent people across the world use herbal medicine for their health care. In the present study, TLC-MS bioautography combined with DPPH, α-amylase and α-glucosidase bioassay were used to compare antidiabetic and antioxidant activities in hydro alcoholic extracts of H. spicatum and also explore in silico molecular docking studies. Materials and Methods: In this paper, hydro-alcoholic extract of H. spicatum was prepared, run TLC and performed TLC- MS bioautography to identify antidiabetic and antioxidant bioactive compounds. Results: HPTLC analysis showed there are 10 and 5 metabolites present at 254 nm and 366 nm respectively in H. spicatum. Further, TLC-MS bioautography studies isolate total two lead compounds such as quercetin and beta-sitosterol. Quercetin shows DPPH, α-amylase and α-glucosidase activity whereas beta sitosterol shows α amylase activity only. Conclusion: In conclusion, TLC-MS bioautography proved to be a good technique to evaluate the antidiabetic and antioxidant potential of plant extracts and will be useful for discovering new therapeutic regimens.| Item type | Current library | Status | Barcode | |
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School of Pharmacy Archieval Section | Not for loan | 2022-1396 |
Background: Traditional system of medicine has been practised since a long time to
cure various diseases. More than 60-80 percent people across the world use herbal
medicine for their health care. In the present study, TLC-MS bioautography combined
with DPPH, α-amylase and α-glucosidase bioassay were used to compare antidiabetic
and antioxidant activities in hydro alcoholic extracts of
H. spicatum and also explore
in silico molecular docking studies. Materials and Methods: In this paper, hydro-alcoholic
extract of
H. spicatum was prepared, run TLC and performed TLC- MS bioautography
to identify antidiabetic and antioxidant bioactive compounds. Results: HPTLC analysis
showed there are 10 and 5 metabolites present at 254 nm and 366 nm respectively in
H. spicatum. Further, TLC-MS bioautography studies isolate total two lead compounds
such as quercetin and beta-sitosterol. Quercetin shows DPPH, α-amylase and
α-glucosidase activity whereas beta sitosterol shows α amylase activity only. Conclusion:
In conclusion, TLC-MS bioautography proved to be a good technique to evaluate the
antidiabetic and antioxidant potential of plant extracts and will be useful for discovering
new therapeutic regimens.
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