Objectives: To design a definite, new and explicit reverse phase high performance liquid
chromatographic (RP-HPLC) method for simultaneous estimation of quantitative and
qualitative curcumin and quercetin in bulk mixture. As per guidelines of International
conference on harmonization (ICH) method was validated. Materials and Methods: In this
study, reverse phase analysis was used to do the analysis Younglin- ACME 9000, C18
(250 x 4.6mm i.d) solvent system methanol and 0.05% orthophosphoric acid as mobile
phase in proportion of 80:20 v/v was used. The flow rate at constant rate 0.7ml/min.
The absorbance of the eluted sample was analyzed by use of UV Visible detector at
254 nm. Results: The proposed improved methodology takes 5.25 min to elute curcumin
and 8.78 min to elute quercetin. The system suitability parameters were studied for the
developed method and were found to be within the acceptable limits. The LOQ and LOD
of curcumin was reported to be 0.041 and 0.081g/ml, respectively, while quercetins
were 0.0048 and 0.039g/ml. Conclusion: The designed methodology validated as per the
ICH guidelines for its linearity, precision, specificity, sensitivity, accuracy, raggedness,
robustness, LOD, and LOQ. The results of the preceding observations show that the
procedure is effective in both qualitative and quantitative analyses of the markers in the mixture.