In the present study, the multiple rectangular and a plus shaped slots microstrip patch antenna (MPA) is designed and its performance has been analysed on the basis of various dielectric constants material such as silicon, silicon dioxide, polyethylene, Teflon, etc. Introduction of slots in MPA is important to find the optimized geometry. In this article, MPA has been designed with nine rectangular slots and one plus shaped slot for multiband application and investigate its accomplishment. The miniaturization has been achieved by inserting the rectangular and plus shaped slots that has been further created on FR4 substrate. The dimensions of this proposed MPA are 26 mm × 18.61 mm and the antenna is uniform from both the sides. The proposed antenna can operate on different frequencies with operating bands of 3.62 GHz for global system for mobile, 5.27 GHz for wireless fidelity (Wi-Fi), and 6.74 GHz for worldwide interoperability for microwave access (WiMAX). The proposed MPA might also be used for certain other broadband applications in the above respective frequencies. Computer simulation technology programme is utilized to characterize the parameters like Voltage Standing Wave Ratio, return loss (S11), surface current, and radiation pattern.