Recent studies have shown that calycosin possess multiple pharmacological properties, containing anti-cancer
activity. Hence, this project explored the effect and possible mechanism of calycosin in pancreatic cancer. After
being cultured, SW1990 cells were exposed to different concentrations of calycosin. 3-(4,5-dimethylthiazol-
2-yl)-2,5 diphenyl tetrazolium bromide, plate colony formation, and transwell experiments assessed cell
proliferation, colony formation, migration, and invasion. Circ_0000285 expression was assessed using
quantitative reverse transcription-polymerase chain reaction experiment. E-cadherin and N-cadherin
protein levels were examined using Western blot. After calycosin treatment, SW1990 cell proliferation,
migration, invasion, circ_0000285 expression, N-cadherin were repressed, and E-cadherin was increased
in dose-dependent way. Circ_0000285 deficiency might hinder cell proliferation, migration, and invasion.
Furthermore, the upregulation of circ_0000285 might partly antagonize calycosin-mediated SW1990 cell
proliferation, migration, and invasion inhibition. Calycosin treatment might block pancreatic cancer cell
development through down-regulating circ_0000285.