Research on the reuse and recycle of plastic waste in building construction offers a way to lessen waste plastic’s damaging impact on the environment and save natural resources. This work aims to find out the best proportion for e-waste concrete with bacteria for best mechanical properties, durability properties and micro structural properties. The flame retardant 4 (FR4) kind of plastic found inside of e-waste is not recyclable or biodegradable. Currently, landfills or incinerators are the methods employed for the elimination of this printed circuit board (PCB) garbage. With this study, researchers want to avoid that and instead make use of that plastic waste for construction applications. In the present case, M25 grade concrete with a w/c ratio of 0.45 is used. Now, to improve the above said properties, the study is conducted with bacteria as additive with the purpose of stopping strength degradation by e-waste concrete. Research work utilizes bacillus subtilis bacteria with different percentages of e-waste replacement. This summary consists of distinct mix proportions cast and tested for different days curing period as 7, 28, 90, and 180 days. The mixes are said to lose weight up to almost 2-13 %, which make a concrete light in weight. Moreover, the self-healing part of the bacteria is also explored. As PCB plastic material reduces the mechanical properties, significant improvement up to 8 % after bacteria induction in concrete have been observed.