Patel, Pinak <br/><br/>
QUANTITATIVE ANALYSIS OF PAMABROM AND IBUPROFEN IN  SYNTHETIC MIXTURE USING  1 ST  ORDER DERIVATIVE SPECTROSCOPY  

 - Vol.11(10) 
 - M P  Innovare Academic Sciences Pvt Ltd  2019 
 - 26-32p. 
<br/><br/> Objective: <br/>The  preliminary  goal  was  to  develop  and  validate  1<br/>st<br/>  order  derivative  spectroscopic  method  for  quantita<br/>tive  analysis  of  Pamabrom <br/>(PAMA) which is a xanthine diuretic and ibuprofen (<br/>IBU) which is a non-steroidal anti-inflammatory age<br/>nt from its synthetic mixture.<br/>Methods: <br/>Analytical  method  was  developed  on  Shimadzu  double <br/>beam  spectrophotometer  equipped  with  UV  probe  2.42 <br/>as  software  using <br/>methanol as solvent. Quantification of PAMA was car<br/>ried out at zero cross over point of IBU that is 29<br/>1 nm and for IBU, it was achieved at 278 nm <br/>which is zero cross over point of PAMA. Method was <br/>validated according to ICH Q2 R1 guidelines. <br/>Results: <br/>Method showed a linear response in the range of 2-1<br/>2 μg/ml of PAMA and 20-120 μg/ml of IBU. Method was<br/> found to be accurate with recovery <br/>between 99.7–100.9 % for PAMA and 100.3–100.7 % for<br/> IBU. The method was found to be accurate and preci<br/>se for quantitative analysis of PAMA and IBU. <br/>Conclusion: <br/>The developed method was successfully validated as <br/>per ICH Q2 R1 guidelines and was successfully appli<br/>ed for quantitative analysis of <br/>a synthetic mixture of PAMA and IBU.  
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS