TY  - GEN
AU  - Sony, Jayaraman
AU  - Variyar, E. Jayadevi
TI  - IMMUNOMODULATORY, ANTICANCER AND ANTIOXIDANT ACTIVI TIES OF  CYCLEA PELTATA (LAM.) HOOK. F. AND THOMSON 
PY  - 2019///
CY  - M P
PB  - Innovare Academic Sciences Pvt Ltd
KW  - PHARMACEUTICS
N2  - Objective: 
The present study was performed to evaluate the imm
unomodulatory, anticancer, and antioxidative proper
ties of the fraction (CP_2) 
isolated from 
Cyclea peltata
.  
Methods: 
Immunomodulation was evaluated in lymphocytes by ly
mphocyte proliferation assay and in THP-1 macrophag
e cell lines by MTT assay. 
The nitrite production by the macrophages was also 
measured by the nitrite assay using griess reagent.
 The anticancer activity of the fraction was 
determined by MTT assay. The antioxidant activity w
as evaluated by DPPH assay and total antioxidant ass
ay by phosphomolybdenum method. It is 
expressed as number of gram equivalent of ascorbic 
acid. 
Results: 
The plant fraction showed the presence of flavonoid
s which induced lymphocyte proliferation rate of 4.
29±0.007 at 100 μg/ml. It was not 
toxic to THP-1 macrophage cells and also could induc
e nitrite production at 1 mg/ml. It also exhibited 
good anticancer activity at 100 μg/ml after 
48h of incubation. The DPPH activity was found to be
 low since 100 μg/ml showed only an inhibition rate
 of 22±0.026. The total antioxidant activity 
at 1000 μg/ml of CP_2 was found to be equivalent to
 79±0.03 μg/ml of ascorbic acid exhibiting moderate
 antioxidant activity.  
Conclusion: 
The fraction CP_2 containing flavonoid, isolated fr
om 
Cyclea peltata
 has good immunomodulatory, antioxidant and antican
cerous property.
UR  - https://innovareacademics.in/journals/index.php/ijpps/article/view/35248/20911
ER  -