Zanwar, Aarti S. <br/><br/>
SIMULTANEOUS ESTIMATION OF MOMETASONE FUROATE AND FORMOTEROL FUMAR ATE  BY HPLC METHOD IN ROTACAPS 

 - Vol.11(2) 
 - M P  Innovare Academic Sciences Pvt Ltd  2019 
 - 12-16p. 
<br/><br/> Objective: <br/>To develop and validate a <br/>simple and sensitive RP<br/>-HPLC method for th<br/>e simultaneous determination of<br/> mometasone furoate<br/> (MOM)<br/> and <br/>formoterol fumarate (FOR) <br/>in pharmaceutical dosage forms.<br/>Method<br/>s: <br/>In <br/>RP<br/>-HPLC  method<br/>,  chromatographic  separation  was  achieved  using<br/>  a  mixture  of  a  solvent  system  consisting  of  methanol<br/>–water  (pH  <br/>3.5) in the ratio of 85:15 % v/v<br/> at a flow rate of<br/> 1 ml<br/>/min <br/>and <br/>detection was carried out at 225 nm. <br/>Result<br/>s: <br/>The run time for the simultaneous estimation of drugs for the proposed method was 1<br/>0 min<br/> as drugs eluted at 5.217 min (MOM) and 8.65<br/>0 <br/>min<br/>  (FOR).<br/>  The  linearity  <br/>was  found  in  the  range  of  33.33<br/>-299.<br/>97 μg<br/>/ml<br/>  and  1<br/>-<br/>9 μg<br/>/ml<br/>  for  MOM<br/>  and  FOR<br/>,  respectively.<br/>  The<br/>  values<br/>  of  limit  of  <br/>detection  and  limit  of  quantification<br/>  were  3.634,  0.266  μg<br/>/ml<br/>  and  11.014,  0.807  μg<br/>/ml<br/>,  which <br/>indicates  the  sensitivity  of  the  method  for  the  <br/>estimation  of  MOM  and  FOR,  respectivel<br/>y.    The <br/>results  of  recovery  studies  for  both  the  drugs  were <br/>within  the  range  i.e.<br/>  98.8<br/>7-101.48 <br/>% <br/>which <br/>indicates  the  accuracy  of  the  method<br/>.  Relative  standard  deviation<br/>  obtained  f<br/>rom<br/>  repeatability<br/>  and <br/>reproducibility  studies  <br/>were <br/>less  than  2<br/>%<br/>indicate<br/>s the <br/>precision of the method.<br/> The <br/>proposed method w<br/>as<br/> validated according to ICH guidelines.<br/>Conclusio<br/>n: <br/>The  proposed<br/>  RP<br/>-HPLC  method  was  found  to  <br/>be  sensitive <br/>and  precise<br/>  because  of  the  low  LOD<br/>,  LOQ  and  %  RSD  values  (<2).  The  <br/>proposed <br/>work <br/>does  not  require  ace<br/>tonitrile  and  ion  pairing  reagent<br/>  as  compared<br/>  to  the  reported  method<br/>s.  Therefore<br/>,  method  can  be  used <br/>preferabl<br/>y for routine analysis due to its simplicity and economic <br/>advantages.  
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS