Reddy, B. Anjaneyulu <br/><br/>
INNOVATIVE METHOD DEVELOPMENT AND FORCED DEGRADATION STUDIES FOR  SIMULTANEOUS ESTIMATION OF  SOFOSBUVIR AND LEDIPASVIR  BY RP HPLC 

 - Vol.11(2) 
 - M P  Innovare Academic Sciences Pvt Ltd  2019 
 - 34-41p. 
<br/><br/> Objective: <br/>To  develop  an  innovative,  rapid,  simple,  cost<br/>-effective<br/>,  stability  indicating  reverse  phase<br/>-high  performance  liquid  chromatography  (RP<br/>-<br/>HPLC) method for simultaneous estimation of ledipasvir (LP) and sofosbuvir (SB) in combination pill dosage form.<br/>Method<br/>s: <br/>The method was developed using C8 column, 250<br/> mm<br/> x  4.6  mm,  5<br/> mm <br/>using mobile section comprising of 0.1%<br/> (v/v)<br/> orthophosphoric <br/>acid  buffer<br/>  at <br/>pH  2.2<br/>  and  acetonitrile  in  the  ratio  of  45:55  that  was  pumped  through  the  column  at  a  flow  rate  of  0.8  ml/min.  Temperature <br/>was <br/>maintained at 30<br/> °C, the effluents w<br/>ere monitored at 260 nm with the help of usage of PDA detector.<br/>Result<br/>s: <br/>The retention time of LP and SB were found to be 2.246 min and 3.502 min.<br/> The approach was found to be linear with the variety of 9<br/>-36<br/>μg<br/>/ml<br/>  and  40<br/>-<br/>240 μg<br/>/ml<br/>  for  LP  and  SB  respectively,  the  assay  of  estimated  compounds  were  found  to  be  99.65%  and  99.73%  w/v  for  LP  and  SB  <br/>respectively.<br/>Conclusio<br/>n: <br/>The  pressured  samples  changed  into  analyzed  and  this  proposed  a  technique  turned  into  determined  to  be  particular  and  stability  <br/>indicating  as  no  interfering  peaks  of  decay  compound  and  excipients  were  observed.  Hence,  the  approach  was  easy  and  economica<br/>l  that  may  be  <br/>efficiently applied for simultaneous estimation of both LP and SB in bulk and combination tablet system 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACEUTICS