TY  - GEN
AU  - Dermane, Affo
AU  - Kepegba, Kafui
TI  - ANTISICKLING ACTIVITY EVALUATION OF FRACTIONS OBTAINED FROM WHOLE EXTRACTS  OF  NEWBOULDIA  LAEVIS  P. BEAUV (BIGNONIACEAE ) 
PY  - 2019///
CY  - M P
PB  - Innovare Academic Sciences Pvt Ltd
KW  - PHARMACEUTICS
N2  - Objective: 
This study aims to 
evaluate the fractions from whole extracts of roots and stem barks of 
Newbouldia 
laevis
and their effect on sickling
. 
Method
s: 
Hydroethanolic 
extracts 
of stem bar
ks and  root
s of 
Newbouldia
 laevis
we
re fractionated by 
the technics of cold precipitation in ethanol. 
The  fractions  obtained  after  phytochemical
  screening  were  subjected  to
  Emmel  test  to  evaluate  their  anti
-sickling  activity.  Active  fractions  were  
tested for DPPH and AAPH assay
 (AAPH induced m
embrane
 lipoperox
idation
 and evaluation of 
reduction of 
hemolysis)
. 
Result
s: 
Two fractions were obtained from each whole extrac
t:    supernatant and pellet fractions.
 Supernatants fractions obtained from whole roots 
bark
s extract and stem barks extract 
at a concentration of 3
0 mg
/ml
 reduced sickling up respectively to 7
% and 
10%
 against 86% for the control
. 
Pellets fractions obtained from 
the both
 extracts induced coagulation of SS blood
 at 30 mg
/ml
 against 86% for the control
. 
Conclusio
n: 
Supernatants fractions of hydroethano
lic whole extract of roots and stem backs of 
Newbouldia 
laevis
 promise as the potential source of 
active molecules against sickle cell disease.
UR  - https://innovareacademics.in/journals/index.php/ijpps/article/view/30112/19151
ER  -