TY  - GEN
AU  - PREMINA, S.
AU  - NIREN ANDREW, S.
TI  - Standardization and application of pcr targeting chlorella species isolated from environmental samples 
PY  - 2021///
CY  - M P
PB  - Innovare Academic Sciences Pvt Ltd
KW  - PHARMACEUTICS
N2  - Objective: Identification of Chlorella species from the environment through 18s ribosomal RNA sequencing. This study was aimed to design primer targeting Chlorella and other closely related algal species targeting 18s ribosomal RNA, ITS1 region.Methods: Sanger sequencing was carried out for the identification of algae up to the genus and species level using an in-house designed primer and optimized PCR conditions.Results: Out  of  2  algae  samples  identified  phenotypically,  one  isolate  identified  as  Chlorellavulgaris  and  other  one  identified  as  Chlorellasorokinianabased on the results of Basic Alignment Search Tool (BLAST).Conclusion: To  conclude,  this  study  provided  primers  with  PCR  conditions  to  characterize  algal  samples  through  molecular  identification  with  100% accuracy than the phenotypic method
UR  - https://innovareacademics.in/journals/index.php/ijpps/article/view/41701/24985
ER  -