TY  - GEN
AU  - Dineva, Alexandrina 
AU  - Angelov, Borislav 
TI  - Development of fast, precise and selective RP-HPLC methods for identification of possible degradation products of ivermectin in isolated rat hepatocytes and in different pH media
PY  - 2022///
CY  - Karnataka
PB  - Association of Pharmaceutical Teachers of India (APTI)
KW  - PHARMACEUTICS
N2  - Aim: A new stability indicating RP-HPLC based methods were developed to identify the
possible degradation products of Ivermectin in isolated rat hepatocytes and in different
pH media using a C18 RP column. Complete validation, including linearity, accuracy,
recovery, precision, robustness, stability, and peak purity, was performed. Materials
and Methods: HPLC system of UltiMateDionex 3000 DAD with LiChrosorb C18 Column
were used in this study. During the investigation, for the removal of interfering fraction
of isolated rat hepatocytes a purification procedure, consisting of protein precipitation
followed by double filtration was developed. Results: Method I was applied effectively
and the results indicated two major hepatic metabolites of Ivermectin. Method II was
developed to monitor the chemical stability in close to physiological conditions. The
appearance of a new peak and the proportional decrease in the sample concentration
indicate a liability in buffer with pH12. Conclusion: The obtained results demonstrated
that the degradation products of Ivermectin in hepatic cells and in alkali media are
different
UR  - https://www.ijper.org/sites/default/files/IndJPhaEdRes-56-2-546.pdf
ER  -