Srinivasan, Vidhya <br/><br/>
Effect of adiponectin on expression of vascular endothelial growth factor and pigment epithelium-derived factor  : An in vitro study 

 - Vol.47(1), Jan-Feb 
 - Mumbai  Wolter Kluwer  2015 
 - 117-120p. 
<br/><br/> This study was carried out to identify the role of adiponectin (APN) in modulating the expression of vascular endothelial growth factor (VEGF) and pigment epithelial-derived factor (PEDF) in relation to ocular angiogenesis.<br/>Materials and Methods: <br/><br/>Human retinal pigment epithelial cell lines (ARPE-19) were cultured in Dulbeco's minimum essential medium with 10% fetal bovine serum (FBS) and exposed to varying concentrations of recombinant adiponectin (5–5 ng/ml) for 1 h. Analysis of VEGF and PEDF mRNA was done by reverse transcriptase and further quantified by quantitative polymerase chain reaction. VEGF and PEDF protein expression were studied using enzyme-linked immune sorbent assay (ELISA).<br/>Statistical Analysis: <br/><br/>Unpaired Student's t-test was used to analyze the significance. P < 0.05 was accepted as statistically significant.<br/>Results: <br/><br/>ARPE cells exposed to APN showed decreased expression of VEGF mRNA, protein whereas PEDF protein is unaltered and PEDF mRNA was increased. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHARMACOLOGY